仿生液晶态凝胶性能对脐静脉内皮细胞功能的影响及其血管化的可行性研究

Vascularization has always been a difficult issues to solve in tissue engineering. The main reason may be that the tissue engineering scaffolds and research conditions in vitro can not meet the human biological microenvironment. The key scientific problem to be solved in this project is to explore the feasibility of biomimetic liquid crystal microenvironment system used for tissue engineering vascularization. The main content of this project is the preparation of ordered fibrin gel, which imitates the structure of vascular tissue and extracellular matrix. This project makes use of the liquid-crystal transformation property of glycine/graphene oxide liquid crystalline to prepare vascularization tissue engineering scaffolds, which based on the research of biocompatibility of liquid crystalline biomaterials for many years. In this project, methacrylated hyaluronic acid was used as main content to fabricate a long-range ordered fibrous tissue engineering vascular scaffold by 3D extrusion bioprinting and UV crosslinking. This project focuses on exploring the structural morphology, conformation, active sites, affinity and the bioactivity of a single VEGF molecule in biomimetic liquid crystal system, as well as to explore the effect of biomimetic liquid crystal microenvironment on the behavior, function and vascularization of HUVECs. The project will lay a theoretical foundation and method for developing a novel liquid crystal vascular tissue repair material that can enhance VEGF activity and promote vascularization of HUVECs.

组织工程血管化一直是组织工程难以解决的关键性问题，其主要原因可能是组织工程支架材料及其体外研发条件无法达到与人体生物微环境相近或相似的要求。本项目拟解决的关键性科学问题就是创新性地探讨用于组织工程血管化的仿生液晶态微环境体系和及其促组织工程血管化的可行性及科学依据。主要内容是在多年液晶态生物材料的生物相容性研究的基础上，利用甘氨酸/氧化石墨烯液晶的液-晶转化性能，制备仿血管组织和细胞外基质的有序纤维凝胶；以丙烯酸酯化透明质酸为基质，借助挤出型生物3D打印和光固化技术，构建高度仿血管的结构特点的长程有序纤维凝胶组织工程血管支架。重点探索单个VEGF分子在高度仿生液晶态体系中的结构形态、构象、活性位点、与受体的亲和力以及生物活性以及仿生液晶态微环境对HUVECs行为、功能和血管化的影响。为开发一种能提高VEGF活性并促进HUVECs血管化的新型液晶态血管组织修复材料奠定理论基础和方法

项目按预期计划执行。本项目在前期液晶态生物材料的生物相容性研究的基础上，利用甘氨酸/氧化石墨烯和甲壳素液晶的液-晶转化性能，制备仿血管组织和细胞外基质的有序纤维凝胶；并借助挤出型生物3D打印和光固化技术，构建仿血管结构特点的长程有序纤维凝胶组织工程支架。重点探索单个VEGF分子在高度仿生液晶态体系中的结构形态、构象、活性位点、与受体的亲和力以及生物活性以及仿生液晶态微环境对HUVECs行为、功能和血管化的影响。为开发一种能提高VEGF活性并促进HUVECs血管化的新型液晶态血管组织修复材料奠定理论基础和方法。