三种入侵植物潜在活性成分的诱导发掘及类冠酮素诱导剂的结构优化研究

Invasive plants generally possessed prominent biological defence capabilities, which implied that they would have great potential for exploring bioactive natural products. Our recent pre-study showed that coronalonoid compound CGM could strongly induce the biosynthesis and accumulation of potential bioactive natural products in three typical invasive plants including W. trilobata. The two major objectives aimed by our this project are the investigation of potential bioactive natural products in these three invasive plants, and the optimization or development of new coronalonoid-based induction method(s). Detailed works in this project will include the study on potential bioactive compounds induced by CGM in these three invasive plants, the structure-activity study of coronalonoid elicitors CR, CGM, INR and IGM on induction of potential bioactive compounds in these three invasive plants, and the further development or optimization of coronalonoid-based induction method(s). It is predictable that the impliment of this research will not only be capable of finding biactive compounds with highly application potential from the three invasive plants, so it can correspondingly actively promote more effective and comprehensive utilization of these harmful plant species, but the new or optimzed induction method(s) that will possibly be achieved in this project would also have the potential to dramatically facilitate exploitation for highly bioactive compounds from more source plants in the future.

入侵植物突出的生物学防御能力暗示它们在发掘生物活性化学成分方面极具潜质。近期我们研究发现新型类冠酮素诱导剂CGM能显著诱导南美蟛蜞菊等三种入侵植物中潜在活性成分的合成积累。本项目将以探索发掘三种入侵植物中具体潜在的生物活性成分并优化获取更具实用性的基于类冠酮素诱导剂的新诱导技术方法为目标主线，具体将利用CGM的显著诱导活性来对三种入侵植物的潜在活性成分实施充分有效地诱导发掘，并基于CGM的结构特征给予的新启示，继而以此三种入侵植物为对象针对已有活性诱导剂CR、CGM以及潜在诱导剂INR和IGM进行类冠酮素诱导剂的诱导-效能构效关系研究，并以此为基础尝试推进基于类冠酮素诱导剂的更优越诱导方法的构建和优化。项目不仅将可望能自三种入侵植物中发掘出具高应用潜质的活性成分以积极推进三种入侵植物的更有效综合利用，而且还将有望获得更具经济实用性的新诱导技术积极推进更广泛资源性植物潜在活性成分的发掘研发。

入侵植物突出的生物学防御能力暗示它们在发掘生物活性化学成分方面具良好潜质。我们本项目基于类冠酮素诱导剂的诱导潜质功能，具体对南美蟛蜞菊等三种入侵植物中潜在的生物活性成分进行了系统地分析发掘，有效地对类冠酮素诱导剂茚酮素和茚酮甘素等进行了合成制备，并总体从三个物种入侵植物中系统地分析解析出了近两百个化合物，其中有近三十个为新化合物，部分成分已被发现具有较显著的抗菌、抗肿瘤细胞毒、抑制糖苷酶等重要生物活性，这为推进三种入侵植物更有效综合开发利用提供了化学物质基础层面确实可靠的依据。另外通过规划设计的功能比较分析研究，明确了几种类冠酮素诱导剂各自在诱导层面的功能特性，揭示冠酮甘素相对于其他几种类冠酮素合成分子于诱导功能上具有潜在优越性，这对夯实基于类冠酮素诱导剂的诱导机制理论和推进类冠酮素诱导剂于植物潜在活性成分发掘方面的更有效应用具有重要意义。