三叶木通潜在活性成分的诱导发掘及诱导机制研究

Alkebia trifoliata, a plant with wide and effective ecological adaptability, has great potential for exploring bioactive natural products. Our recent pre-study showed that coronalon (CR), a structural and functional mimic of JA-Ile, strongly induced the biosynthesis and accumulation of potential bioactive compounds in A. trifoliata. While En-Val, a structurally similar compound to CR, did not like CR to mimic the function of JA-Ile, but rather induced a differenct spectrum of chemicals in A. trifoliata. This indicated that an unkown signaling pathway paralel to JA-Ile signaling would exist in A. trifoliata, which regulate the biosynthesis of part of the potential bioactive compounds in this plant, possibly taking advantage of JA-Val or another JA-AAs as signaling molecule. In this project, we will manage to identify the structures and bioactivities of those potential bioactive chemicals induced by CR or EI-Val, by using a combination of phytochemical, spectroscopical and analytical techniques. We will also manage to identify endogenous JA-AAs (JA amino acid conjugates) of A. trifoliata and further try to analysis their induction activities or functions by using GC-MS and LC-MS techniques. It is predictable that the identification of highly valued and inducible biactive compounds from A. trifoliata will promote a more effective utilization and development of this medicinal plant. It is also predictable that the clarification of JA-AAs and their activities in A. trifoliata will well contribute the understanding of a more clear regulation network for plants to regulate the biosynthesis of their potential bioactive chemical constituents.

三叶木通在发掘潜在生物活性成分方面潜质优越。研究显示冠酮素CR能有效诱导三叶木通潜在活性成分的合成积累，但具体成分有待发掘。另发现CR的近似物EI-Val未能有效模拟JA-Ile但却明显诱导另外未知成分的合成，因而我们推断该植物存在旁路于JA-Ile并可能基于其他JA-AAs的调控潜在活性成分合成积累的未知信号通路。本项目将利用CR及En-Val的显著诱导活性来对三叶木通潜在活性成分实施充分的诱导发掘，并将利用气质和液质等技术系统分析三叶木通内源JA-AAs及其相比于EI-Val的诱导活性与功能，据此获得该植物基于其他JA-AAs分子激活旁路于JA-Ile的未知信号通路的可靠证据。本项目将能自三叶木通中发掘出具高应用潜质的活性成分以积极推进该植物更有效综合开发利用，并将有望揭示三叶木通调控其部分潜在活性成分合成积累的新机制，从而为完善植物调控潜在活性成分合成积累的网络机制系统提供科学依据。

三叶木通具有潜在丰富的生物活性成分有待发掘，而CR等冠酮素类诱导剂能诱导合成积累三叶木通的潜在活性成分进而可促使其发掘，但相关信号机制待解析。我们本项目基于类冠酮素诱导剂的诱导潜质功能，具体对三叶木通植物中潜在的生物活性成分进行了系统地分析发掘，总体从三叶木通植物中系统地分析解析出了近百个化合物，其中有三十三个为新化合物，部分成分已被发现具有较显著的抗菌、抗肿瘤细胞毒、抑制糖苷酶等重要生物活性，这为推进该植物更有效综合开发利用提供了化学物质基础层面确实可靠的依据。另外通过规划设计的信号分子鉴定与功能比较分析研究，揭示了三叶木通中存在对应于En-Val诱导活性与功能的潜在JA-AAs信号分子并明确了其信号时效动态变化等功能特征，此进展结果呈现了三叶木通调控其潜在活性成分合成积累的信号多样性，对于推进完善植物调控潜在活性成分合成积累的网络机制系统具有重要价值和意义。