壮医药线点灸疗法对围绝经期模型大鼠雌激素及其受体的HPOA调控效应研究

Medicated Thread Moxibustion of Zhuang Traditional Medicine has been included in the national non-material cultural heritage list.Its warm and efficacy of the instantaneity of superficial acupuncture effect obvious effect, different from classical acupuncture points ，but medicine line mechanism research is relatively weak。The treatment in regulating Perimenopausal Perio Syndrome (PPS) related symptoms have better effect. Group pilot study confirmed by improve serum levels of Estradiol (E2)   and uterine estrogen receptor α (ER-α) expression to play a role, in order to explore the effect of therapy in the treatment of the existence effect values change with time, whether the mechanism and the hypothalamus -pituitary - ovarian axis (HPOA) ER/ER mRNA related regulation effect? As a result,the project to a 4 - ethylene vinyl ring (VCD) to the model of perimenopausal rabbits as the research object, in different periods Medicated Thread Moxibustion of Zhuang Traditional Medicine intervention for the observation point, through the observation of appearance manifestation and pathological morphology observation of combining the examination way, from the work time, the peak effect explore its estrogen receptor (ER) effect in HPOA temporal variation used RT-PCR, immunohistochemistry and Western Blot techniques and approaches, such as detection of the perimenopausal rabbits hypothalamus, pituitary, ovary ER and ER mRNA expression changes, and to explore the treatment regulation of estrogen receptor effect mechanism of PPS, and looking for the treatment of the optimal timing of treatment, lay a foundation for its further study.

壮医药线点灸疗法（药线）已列入国家级非物质文化遗产名录，其瞬时性温热与药效的浅表穴位效应明显区别于经典针灸的穴位效应，但药线机制研究较薄弱。前期课题组应用药线可 改善围绝经期综合征（PPS）患者临床症状，并首次证实可提高血清E2水平及子宫ER-α表达，由此，为了深入明确其在治疗中是否存在雌激素效应值的时序变化规律，探索其机制是否与下丘脑-垂体-卵巢轴（HPOA）ER/ER mRNA调控效应相关？为此，本项目以4-二氧化乙烯基环己烯（VCD）致围绝经期模型大鼠为研究对象，以药线不同干预时段为观察点，通过外观表征、细胞涂片、血清E2观察相结合的方式，探寻其雌激素时序变化规律；应用RT-PCR、免疫组化、Western Blot等技术与方法，检测VCD大鼠下丘脑、垂体、卵巢ER及其ER mRNA表达与含量，探讨药线调控PPS的HPOA雌激素效应机制，寻找药线的最佳治疗时机，为其深入研究奠定基础。

围绝经期综合征属于壮医“虚病”范围，壮医认为妇女经、孕、产、乳都是以“勒”（血）为用，而人体生命是以“嘘”（气）为用，妇女进入围绝经期，生殖机能衰退，勒”（血）虚、“嘘”（气）虚，龙路火路气机不畅，成为了PPS发病的主要原因。壮医药线点灸疗法具有悠久的历史且对临床上一些常见病多发病等均有较好疗效。根据临床经验及文献研究，本实验选取善调龙路、火路的壮医经验穴位脐周穴、下关元和背八穴，以期达到通调龙路，健运谷道、水道，养心的功效。.通过本研究证明壮医药线点灸疗法能提高血清中E2水平，降低LH、FSH水平且在一定时段内治疗时间与治疗效果呈正相关关系，揭示壮医药线点灸疗法的量效关系与最佳治疗时机；壮医药线点灸疗法能提升下丘脑、垂体和卵巢组织中ER蛋白和ER mRNA基因表达量，该疗法能够调控下丘脑-垂体-子宫-卵巢生殖-内分泌轴（HPOA），明确了药线在HPOA中ER/ER mRNA调控效应；壮医药线点灸疗法能显著提高围绝经期模型大鼠血清中IL-2和IFN-γ水平，降低IL-4和IL-10的水平，其机制可能与调节机体内分泌-免疫系统有关。壮医药线点灸疗法能改善卵巢功能，缓解围绝经期综合征出现的多种症状，起到调理围绝经期综合征的作用。.完成相关论文及人才培养。.（1）参加国内外学术交流4次；.（2）在国际和国家级学术刊物上发表研究论文7篇，其中SCI论文1篇，科技核心期刊2篇；.（3）培养硕士研究生6名，本研究结果在中国知网硕士论文在线登出1篇。