生存运动神经元蛋白与转录因子IIF的RAP30

Object: To study the binding of the SMN protein to the general transcription factor IIF in vitro. Method: Full length SMN protein, SMN truncated mutants D4, D3, D2 were expressed respectively using the GST fusion protein system. Pull-down assay in vitro and Western Blot were used to detect the binding of SMN protein and TFIIF. Results: Binding of the SMN protein and RAP30 was confirmed in vitro. Conclusion: These findings indicate an association of the SMN protein with TFIIF and suggest a role for the SMN protein in the RNA polII transcription/processing machinery.

最新的研究表明脊髓肌肉萎缩症的致病基因的产物SMN蛋白和转录因子IIF的RAP30亚基均与RNA聚合酶II结合。本课题旨在通过重组蛋白在体内和体外的结合试验探讨SMN与RAP30间的相互作用；通过丙氨酸扫描法鉴定出介导SMN与RAP30结合的关键氨基酸；通过转录功能试验及稳定转染细胞系的建立研究SMN与RAP30的结合在基因转录调控及运动神经元的生存中的作用.