轴向配体对微过氧化酶电化学和电催化行为的影响

In the recent years, the sduty of the electrochemical reactions of biomacromolecules begins to investigate the effect of the changes in the structure of the biomacromolecules on their electrochemical properties. In this project, the effects of the interaction of MP-11 or MP-8, as the model coumpound of proteins and enzumes containing heme with the axial ligand or heavy metal ion, including the rare earth metal ionon the secondary structure, electrochemical and electrocatalytic properties were investigated. This kind of studies would clearly understand the effect of the structures of the heme and the pepertide in proteins and enzumes containing heme on their electrochemical and electrocatalytic properties. It can give more information about the effect of biomacromolecular structure on the electrochemical prop[erties. Especially, it can make the strong base for developing new biological and biomimic materials and for understanding the mechanism of the use of the rare earth elements in the agriculture. It was found that MP-11 can be modified on the electrode surface with polyimide, Nanfion membrane and nylon membrane. These modification methods are easy and do not lead to the denaturation of MP-11. When the sixth ligand of heme in MP-11 exists, generally, the formal redox potential would be shifted to the negative direction and the electrocatalytic activity would be decreased. It was suggested that the heavy metal ion (including rare earth ion) firstly interact with -C=O in carboxyl groups of heme in MP-11 or MP-8. On the one hand, the interaction makes the increase in the non-planarity of heme and the decrease in the density of the electron cloud. On the other hand, the content of the random structure is decreased and the content of the regular structure is increased. Therefore, the reversibility of their electrochemical reactions and the electrocatalytic activity are increased.

在用适当的方法把微过氧化酶固定在电极表面和用电化学方法制备成五或四配位的微过氧化酶的基础上，研究不同轴向配体对微过氧化酶电化学和电催化性能的影响，加上对不同微过氧化酶和不同环境影响的研究，就能了解影响微过氧化酶电化学和电催化性能的主要因素，这对理解生物分子之间的电子迁移机理以及研究生物和仿生电催化剂创造了条件。