解析链格孢菌诱导棉花叶片衰老过程中基因的转录调控及信号通路变化

It is a serious problem for the influence of premature senescence on cotton production in China. In view of the previous work, we firstly revealed that the key factors for causing of cotton premature senescence is because of compound chilling stress and infection of Alternaria leaf spot pathogens Alternaria alternate. This finding also provided a new field of vision for studying and revealing the key causes of cotton premature senescence with compound of physiological and pathological factors. This study aims to further reveal the key regulated genes, signal pathways and its regulation function in priming and developing of cotton leaves senescence process, which is induced by compound physiological/pathological factors. This study will be helpful for further revealing the molecular mechanisms of cotton leaf senescence induced by compound chilling stress and infection of Alternaria leaf spot pathogens, also would be helpful for providing the foundation and basis for further exploration and establishment of cotton premature senescence prevention and control technology.

早衰是严重危害我国棉花生产的重要科学问题。鉴于在前期工作中我们已率先揭示了低温复合链格孢菌侵染是引起棉花轮纹斑病，造成棉花叶片细胞死亡，最终导致棉花早衰的关键成因。该现象的阐明,也开拓了从生理和病理复合因素这一全新的视野来研究和揭示棉花早衰成因。本研究旨在进一步揭示这一生理/病理复合因素引起轮纹斑病发生，导致棉花叶片衰老过程中，棉花基因的转录调控特征及信号通路变化，确定其中的关键调控基因和信号通路，明晰它们与棉花叶片衰老死亡间的内在关系。这有助于从分子水平上进一步揭示低温及链格孢菌这一生理/病理复合因素引起棉花叶片衰老，导致棉花早衰的的分子机理，同时也为进一步探索和创建棉花早衰防控技术提供基础和依据。

链格孢侵染引起的棉花早衰严重危害我国棉花生产。揭示链格孢诱导棉花叶片衰老过程中的基因转录调控和信号调控途径，有助于揭示棉花抗链格孢的分子基础，为防控棉花早衰提供可行的策略和技术方法。本研究分别从抗病基因、促分裂原活化蛋白激酶级联信号通路(MAPKs)及棉花次生代谢途径这三个层面解析这一过程的分子基础。研究明确香豆素合成途径关键酶基因Ghpal、Ghc4h 和Gh4cl 在棉花抗链格孢菌中的作用。明确了促分裂原活化蛋白激酶级联信号通路(MAPKs)及棉花CC-NBS-LRR抗病基因在棉花抗病中的作用。在揭示了链格孢菌侵染是导致棉花早衰发生危害关键因子的基础上，创建棉花早衰综合防控技术体系，从而为我国棉花生产提供了有力地保障。有关工作荣获中国植物保护学会科技进步二等奖1项；施普林格科学出版社出版的英文学术专著独立章节一篇；中国科学等核心期刊发表学术论文7篇；培养硕士研究生6名。