大豆抗斜纹夜蛾的生理生化机制研究

The cotton worm is a very important leaf-feeding insect species of soybean, especially in southern China，soybean plant are seriously damaged, and limiting soybean produetion. Supported by the National Natural Science Foundation of China, we have identified the resistant soybean species, and the mechanism, inheritance, molecular marker of soybean resistant to cotton worm have bean studies. In this project, continually, physiological and biochemical mechanism of the response of soybean tocotton worm will be studied. We will study the activity changing patterns of nutritional components, enzymes, plant hormones and plant secondary substance relate to response of soybean to cotton worm.The out put of this project will provide new insights into resistance of soybean tocotton worm from both physiological and biochemical Mechanism viewpoints, and give literature references to gene mining, genetic improvement and plant breeding for soybean resistant to cotton worm.

斜纹夜蛾是重要的大豆食叶性害虫，特别是南方大豆产区发生危害严重，造成产量的严重损失。在前两个国家自然科学基金的资助下，开展了大豆对食叶性害虫的抗性鉴定、抗性机制、抗性遗传和分子标记等研究。本项目将在已有研究工作基础上，开展大豆抗斜纹夜蛾的生理生化机制研究。分析抗、感大豆材料之间和大豆在受到斜纹夜蛾的为害诱导下，相关性营养物质、酶、激素、次生物质的变化规律，研究大豆抗斜纹的生理生化机制，为挖掘大豆抗斜纹夜蛾基因、创制抗虫种质和实现大豆抗斜纹夜蛾高效分子育种提供理论依据。

斜纹夜蛾是重要的大豆食叶性害虫，特别是南方大豆产区发生危害严重，造成产量的严重损失。在本项目的资助下，开展了大豆抗斜纹夜蛾的生理生化机制研究。分析抗、感大豆材料之间和大豆在受到斜纹夜蛾的为害诱导下，相关营养物质、酶、激素、次生物质的变化规律，研究大豆抗斜纹蛾的生理生化机制，为挖掘大豆抗斜纹夜蛾基因、创制抗虫种质和实现大豆抗斜纹夜蛾高效分子育种提供理论依据。. 从分析的生理生化物质结果来看，（1）高抗与高感品种之间：高抗品种赶泰-2-2超氧化物岐化酶活力总体水平上比高感品种皖82-178高；不管是接虫前还是接虫后，高抗品种的三种激素水平都高于高感品种。（2）在接虫斜纹夜蛾为害诱导后，大豆叶片的总糖含量、蛋氨酸含量、超氧化物岐化酶（SOD）活性、过氧化氢酶（CAT）活性、多酚氧化酶（PPO）活性、乙烯（ETH）含量、苿莉酸（JA）含量、脱落酸（ABA）含量都显著增加，说明这些物质在受到斜纹夜蛾为害过程中参与了抗虫防御反应，其作用机理值得进一步深入研究。