基于表面聚合厚度可控分子印迹涂层的肿瘤标志物选择性识别研究

In recent years, being a facile method for the preparation of high specific and very stable polymer, molecular imprinting technique attracts wide interests of researchers. In the proposal, we explore a dip-coating photo-polymerization using silicane material as substrate and iniferter as living initiator. In this vein, polymer coatings with controllable thickness will be prepared in automatic and fast way, which dissolves the problems such as tedious process involved, time consuming, expensive, less controllable, heterogeneous surface brought by traditional coating method. Next, the coating method developed here will be combined with molecular imprinting technique to prepare high selective imprinted coating, using the tumor markers as template and applying both molecular modeling and non-imprinted polymer library for the screening of monomers, for solid phase microextraction and sensor membrane with predetermined thickness. After that, we couple the imprinted microextraction with liquid phase micro extraction to develop high selective and green extraction technique with high enrichment factor and the technique will be applied for the sample preparation of complex body fluids. Besides, molecular imprinted fluorescent sensor will be developed based on the fluorescent property of analytes and selective recognition provided by imprinted membrane. This project is an instant deepening and broadening of our previous study.

近年来，分子印迹技术作为一种制备高特异性、高稳定性聚合物的方法，引起了广泛的关注。本项目拟探索以SiO2基材料为载体，用活性自由基引发剂，浸渍提拉光聚合的方法，从而自动、快速、可控制备预期厚度聚合物涂层，解决传统方法过程繁琐、耗时、昂贵、涂层不均一、可控性差等问题。进而，所建立的涂层制备方法与分子印迹技术结合，以5-羟色胺和5-羟基吲哚-3-乙酸等小分子生物标志物为模板，利用分子模拟软件和非分子印迹聚合物库筛选最佳功能单体和交联剂，制备高选择性、厚度可控分子印迹固相微萃取（SPME）涂层和印迹敏感膜。再次，将石英纤维分子印迹SPME与液相微萃取结合，研制具有高选择性、高富集倍数、绿色、快速萃取体系用于体液等复杂体系的样品前处理；以模板分子的荧光性质为基础，基于印迹敏感膜的选择性识别，发展分子印迹荧光传感器。本项目是课题组对已从事课题的及时深入和扩展。

近年来，分子印迹技术作为一种制备高特异性、高稳定性聚合物即 “塑料抗体”的方法，引起了广泛的关注。本项目以玻璃片为载体，浸渍提拉光聚合和载体膜三明治法制备聚合物涂层的方法，快速、可控制备预期厚度聚合物涂层，解决传统方法耗时、昂贵、涂层不均一、可控性差等问题。进而所建立的涂层制备方法与分子印迹技术结合，以2,4-二氯苯氧乙酸等小分子生物标志物为模板，制备高选择性、厚度可控分子印迹固相微萃取涂层和印迹敏感膜。将中空纤维膜保护微固相萃取与液相微萃取结合，研制具有高选择性、高富集倍数、绿色、快速萃取体系用于复杂体系的样品前处理。本项目研究的厚度可控聚合物涂层和中空纤维膜保护微固相萃取装置制备简单、萃取效率高，绿色的微萃取方法具有广阔的应用前景，需要进一步拓展研究。