3D生物打印重塑大鼠阴道

Three-dimensional (3D) bioprinting is a new technology in the late 80s, 20th century. Compared with the traditional tissue engineering techniques, the biggest advantage of 3D printing is that it provides accurate and fast scaffold integrative construction with complex macroscopic configuration and internal microstructure, it can realize individual production for certain patients and certain tissues and organs. This research will first induce bone marrow mesenchymal stem cells (BMSCs) which have multiple differentiation potential and taken form GFP transgenic rats to vaginal epithelial cells, smooth muscle cells, fibroblasts and vascular endothelial cells. Prepare acellular porcine vaginal matrix hydrogel. Using stereology method for rat vaginal reconstruction and measuring the volume of rat vaginal epithelial layer, connective tissue layer and muscular layer and the numberical density(Nv) of each layer. Then we will 3D print the rat vagina in vitro, test it to be qualified and transplant it to reconstruct the rat vagina compared to the reconstructed vagina with the traditional tissue engineering techniques in morphology and function. To explore the better technique of vaginal reconstruction.

3D 打印是 20 世纪 80 年代末兴起的一门新技术，与传统组织工程技术相比，3D 打印的最大优点在于其能够精确、快速实现支架复杂的宏观外形与内部微细结构的一体化构建，可以实现针对特定患者、特定组织器官的个性化生产。本研究首先将取自GFP转基因大鼠的具有多向分化潜能的BMSCs定向诱导分化为阴道上皮细胞、平滑肌细胞、成纤维细胞和血管内皮细胞；制备猪阴道脱细胞基质水凝胶；应用体视学方法3D重建大鼠阴道，测量大鼠阴道上皮层、结缔组织层和肌层的体积和各层所含细胞的数密度，而后体外3D打印大鼠阴道，检测合格后植入大鼠体内与传统的组织工程技术构建的大鼠阴道进一步进行形态和功能学的比较。探索更优的阴道重建技术。

3D打印是20世纪80年代末兴起的一门新技术，与传统组织工程技术相比，3D打印的最大优点在于其能够精确、快速实现支架复杂的宏观外形与内部微细结构的一体化构建，可以实现针对特定患者、特定组织器官的个性化生产。本研究首先将大鼠的具有多向分化潜能的BMSCs定向诱导分化为阴道上皮细胞、平滑肌细胞、成纤维细胞和血管内皮细胞，并分析其可能的诱导分化机制；制备猪阴道脱细胞基质水凝胶，并对其进行检测和改进；应用体视学方法测量大鼠阴道上皮层、结缔组织层、肌层和外膜层的厚度和主细胞层所含细胞的体密度，而后体外3D打印类大鼠阴道组织，检测合格后植入大鼠体内与传统的组织工程技术构建的大鼠阴道进一步进行形态和功能学的比较。探索更优的阴道重建技术，为3D打印阴道的临床转化奠定基础。