消肿止痛合剂对皮瓣修复后血管再生及其信号通路VEGF-Dll4/Notch的机制研究

Skin flap is a major trauma and orthopedic important means of repair, but the skin flap necrosis, set up the circulation of the blood is the key to the flap survival as soon as possible. VEGF - Dll4 / Notch signaling pathway plays an important role in angiogenesis, become a research focus in recent years, but has not seen in promoting skin flap angiogenesis such reports and promoting angiogenesis, clinical in exogenous invasive methods such as vascular growth factors. In recent years, Chinese medicine made certain research progress in this area. Previous studies have found that Xiaozhong zhitong mixture of rat tail replantation after establishing blood circulation has good effect, this topic based on this, puts forward assumption: Xiaozhong zhitong mixture can make VEGF - Dll4 / Notch signaling pathway in the process of angiogenesis accurate expression, can produce quantity and good quality of blood vessels. Using animal model, the cell model, this study with Xiaozhong zhitong mixture intervention, through the observation in the rat model of their new blood vessels, in vitro cell culture, molecular biology, such as index, after adopting specific blocker, to observe the effects of Xiaozhong zhitong mixture of blocking the path, to study the effect of acetanilide detumescence mixture of flap vascular regeneration mechanism and targets, flap survival is dedicated to provide you with a new idea and new method.

皮瓣是重大创伤和整形外科重要的修复手段，然皮瓣坏死屡见不鲜，尽快建立血液循环是皮瓣成活的关键。VEGF-Dll4/Notch信号通路在血管再生中发挥着重要作用，成为近年来研究的热点，但在促进皮瓣血管再生方面却未见此类报道。促进血管再生，临床多采用血管生长因子等外源性有创手段。近年来，中医药在这方面的取得了一定的研究进展。前期研究发现，消肿止痛合剂能促进鼠尾再植后的血管修复，提高VEGF表达水平。本课题在此基础上，提出假设：消肿止痛合剂能够使VEGF-Dll4/Notch信号通路在血管再生过程中精确统一表达，能够产生数量多且质量好的血管。本研究借助动物模型、细胞模型，以消肿止痛合剂干预，通过观测大鼠模型皮瓣新生血管形态学、体外细胞培养分子生物学等指标，后采用特异性阻断剂，观察消肿止痛合剂对阻断通路后的影响，探讨消肿止痛合剂对皮瓣血管再生的作用机制及靶点，旨在为皮瓣成活提供一种新思路和新方法。

皮瓣是重大创伤和整形外科重要的修复手段，广泛应用临床，效果满意，然皮瓣坏死屡见不鲜，坏死的病例并不少见，成为手术失败的主要原因之一，尽快建立血液循环是皮瓣成活的关键。VEGF-Dll4/Notch信号通路在血管再生中发挥着重要作用，成为近年来研究的热点，但许多方法并未在临床中得到应用。因此如何有效解决皮瓣坏死的问题仍然任重道远，缺血再灌注损伤是皮瓣坏死的主要原因。前期研究发现，消肿止痛合剂能促进鼠尾再植后的血管修复，提高VEGF表达水平。本课题在此基础上借助动物模型、细胞模型，以消肿止痛合剂干预，通过观测大鼠模型皮瓣新生血管形态学、体外细胞培养分子生物学等指标，后采用特异性阻断剂，观察消肿止痛合剂对阻断通路后的影响研究消肿止痛合剂能够使VEGF-Dll4/Notch信号通路在血管再生过程中精确统一表达，能够产生数量多且质量好的血管，实验过程完成顺利，取得了理想的效果。研究消肿止痛合剂对皮瓣血管再生的作用机制及靶点，旨在为皮瓣成活提供一种新思路和新方法，具有广泛的临床应用前景，同时该课题是在前期研究的基础上进一步深入探索，我们的猜测和理论对皮瓣外科的进一步发展具有重要的推动作用。