固脱注射法治疗盆腔器官脱垂重建盆底支持系统的动物实验研究

Pelvic organ prolapse is a kind of common、 frequently-occurring disease affecting women's physical and mental health and quality of life. The Strengthening Injection Therapy with less pain, short course, less cost, repeatable application advantages is restricted for its mechanism of action, security and concentration-response relationship. We will use 36 beagle, take bao model method of pelvic organ prolapse, adopt more clinical xiaozhiling injection around the prolapsed organs. According to the size of injection dose, we divide into 6 groups, each group of six dogs, preoperative and postoperative 1, 5, 10 days ear venous blood were taken to determine the WBC, BUN, Cr, ALT, AST. We will observie animals living conditions, the morphological changes of pelvic prolapsed organs. We kill animals in 15 days, observe prolapsed organ morphology and its relationship with the surrounding tissues, take pathologic examination, to observe the morphology of pelvic floor muscles of pelvic floor tissue changes, the proportion of muscle fiber types and the diameter of the fibers changes; The content of collagen, Ⅰ and Ⅲ proportion of collagen fibers, form, structure, the change of solubility, the expression of RAGE, collagen Ⅰ, MMP-1, MMP-3, LOX, TIMP, TIMP-3, TGF-β1; Fibroblast morphology, cell structure, the structure of the cytoskeleton morphology, cytoskeleton and beta actin cytoskeleton proteins, β-tubulin and vinculin content changes. We will clearly know the concentration-response relationship and safety. We will do the research from muscles, general pathology, pelvic connective tissue, cells, molecules, mechanics, concentration-response relationship and safety for the prevention and treatment of pelvic organ prolapse.

盆腔器官脱垂（POP）是临床常见病、多发病，固脱注射法具有痛苦小、疗程短、花费少、可重复应用的优点。我们以36条比格犬，采取包氏法造成人工POP模型，采取临床上较为规范的消痔灵周围注射疗法进行治疗，并根据注射剂量大小分为6组，每组6条犬，术前与术后第1、5、10天分别取耳静脉血测定WBC、BUN、Cr、ALT、AST，观察动物一般生存状态、盆腔器官脱垂的形态学变化。第15天后处死动物，观察脱垂器官形态及其与周围组织的关系，并取注射周围组织送病理检查，观察盆底肌的形态学、肌纤维类型的比例与直径变化；胶原蛋白的含量、Ⅰ型及Ⅲ型胶原纤维比例、形态、结构、溶解性的改变；盆底结缔组织MMPs、TIMPs等的表达变化；成纤维细胞形态、结构、细胞骨架形态、结构及骨架蛋白的含量变化；从盆底肌一般病理学、细胞、分子、细胞力学角度研究固脱注射法治疗POP的机制及本病发病机制，为POP的预防和治疗开拓新思路。

1盆腔器官脱垂（POP）是临床常见病、多发病，固脱注射法具有痛苦小、疗程短、花费少、可重复应用的优点。我们以6条比格犬+36只巴马猪，建立人工POP模型，采取临床上较为规范的消痔灵周围注射疗法进行治疗，在正式实验阶段根据注射剂量大小分为6组，每组5只巴马猪，术前与注射后第1、3、7天分别取前腔静脉血测定WBC、 ALT、AST、UREA、Cr，观察动物一般生存状态、盆腔器官脱垂的形态学变化。第7天后处死动物，观察脱垂器官形态及其与周围组织的关系，并取注射周围组织送病理检查，观察盆底肌的形态学、肌纤维类型的比例与直径变化；胶原蛋白的含量、Ⅰ型及Ⅲ型胶原纤维比例、形态、结构、溶解性的改变；盆底结缔组织MMPs、TIMPs等的表达变化；成纤维细胞形态、结构、细胞骨架形态、结构及骨架蛋白的含量变化；从盆底肌一般病理学、细胞、分子、细胞力学角度研究固脱注射法治疗POP的机制及本病发病机制，为POP的预防和治疗开拓新思路。