两亲生物活性肽的自组装、界面性质及功能性输送研究

Self-assembly of amphiphilic bioactive peptides can construct ordered microstructures and is an important pathway for the development of modern functional foods. In this study, amphiphilic peptides with specific bioactivity are prepared by limited enzymatic hydrolysis and further purified, identified and synthesized. Self-assembly of active peptides are then regulated for the preparation of polymorphous peptide-based assemblies with functional loading capacity. Furthermore, the above mentioned peptide assemblies are used as building blocks for the preparation of multi-structured emulsions, designed from the view of hierarchical structure with the purposes of controlling lipid digestion. The research contents mainly include: (1) Directional preparation, synthesis and conformational characterization of amphiphilic active peptides; (2) Behavior regulation, characterization, and functional delivery of self-assembled amphiphilic bioactive peptides. Peptide assemblies with polymorphous states (i.e. particles, fibers, and gels, etc.) are then prepared and designed for functional delivery by co-assembly technique. (3) Controlled behavior of the bioactive peptide assemblies at interfaces and the regulation of lipid digestion in emulsion systems. Prepared peptide assemblies are further used as building blocks for a rational design of food emulsion system where emulsion structural changes as well as lipid digestion kinetics are particularly emphasized when underwent in vitro gastric-intestinal tract, aiming to figure out the regulation mechanism of the interfacial properties on emulsion stability and lipid digestion. This project aims to reveal the association between the self-assembly behavior of peptides and their bioactivity, interfacial activity and functional delivery capacity, which will further provide new ideas and theoretical guidance for the development of new functional foods and food ingredients

两亲生物活性肽的自组装可构建有序的微结构，是现代功能食品研发的重要途径。本研究利用限制性酶解技术，以功能活性为导向制备并合成两亲生物活性肽，通过调控活性肽的自组装行为制备多形态且具有功能性荷载能力的肽自组装体，进一步利用层次结构设计原理构建多类型乳液结构，以实现脂质消化的可调控。研究内容主要包括：（1）两亲活性肽的定向制备、合成及构象表征；（2）两亲活性肽的自组装、组装体结构表征及功能性输送。构建包括颗粒、纤维、凝胶等多形态的肽组装体，采用共组装技术荷载多种疏水性功能成分；（3）活性肽自组装体的界面行为及乳液脂质消化特性的调控研究。以活性肽组装体为组件进一步构建乳液体系，重点监测消化过程乳液结构的变化及脂肪消化动力学，研究其界面特性对乳液稳定及脂质消化的调控机制。本项目旨在揭示肽的自组装行为与其生物活性、界面活性及功能性输送之间的关联，为新型功能食品及食品配料的开发提供新理念和新方法。

两亲生物活性肽的自组装可构建有序的微结构，是现代功能食品研发的重要途径。本研究利用限制性酶解技术，以功能活性为导向制备并合成两亲生物活性肽，通过调控活性肽的自组装行为制备多形态且具有功能性荷载能力的肽自组装体，进一步利用层次结构设计原理构建多类型乳液结构，以实现脂质消化的可调控。研究内容主要包括：（1）两亲活性肽的定向制备、合成及构象表征；（2）两亲活性肽的自组装、组装体结构表征及功能性输送。构建包括颗粒、纤维、凝胶等多形态的肽组装体，采用共组装技术荷载多种疏水性功能成分；（3）活性肽自组装体的界面行为及乳液脂质消化特性的调控研究。以活性肽组装体为组件进一步构建乳液体系，重点监测消化过程乳液结构的变化及脂肪消化动力学，研究其界面特性对乳液稳定及脂质消化的调控机制。本项目旨在揭示肽的自组装行为与其生物活性、界面活性及功能性输送之间的关联，为新型功能食品及食品配料的开发提供新理念和新方法。