TLR9信号途径在成牙本质细胞天然免疫反应及DSPP基因表达调控中作用的研究
基本信息
结项摘要
成牙本质细胞是形成牙本质，也是牙髓最先接触细菌微生物的细胞。TLR9是特异识别含未甲基化CpG基序的细菌DNA的天然免疫受体，在许多组织细胞免疫防御反应中发挥关键的作用。DSPP 主要由成牙本质细胞表达，在牙本质形成和矿化中发挥关键的作用。本项目拟通过小鼠切牙牙片培养、实时定量PCR、蛋白杂交、RNA干涉、报告基因分析和EMSA等手段研究TLR9介导成牙本质细胞天然免疫反应及调控DSPP基因表达的细胞内信号途径，寻找DSPP基因启动子上TLR9信号途径结合位点，并确定与之相结合的转录因子。旨在阐明TLR9介导成牙本质细胞免疫反应和调控DSPP基因表达的分子机制，探讨龋病进展过程中，细菌DNA感染、成牙本质细胞免疫反应、DSPP基因表达与第三期牙本质形成相互间的联系，对阐明牙髓病病因、牙髓损伤修复的分子机制和DSPP基因表达调控规律有重要意义，为免疫防龋研究和活髓保存治疗提供新的思路和途径。
项目摘要
项目成果
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数据更新时间：2023-05-31
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