花生钙调素结合蛋白S-腺苷甲硫氨酸合成酶1介导钙离子调控分枝发育分子机理的研究

The number of branches is an important botanical character of peanut, and it has a positive correlation with the yield of peanut. It is the main factor contributing to the high yield of peanut. At present, there were many studies on the growth and development of branches in the model plants such as Arabidopsis thaliana and rice, but little was known in peanut. A new calmodulin binding protein, S-adenosylmethionine synthase 1 (SAMS1) was identified and the number of tobacco branches that overexpressed this gene increased significantly. Control peanut seedlings, transgene AhSAMS1and CaM4-AhSAMS1 tobacco lines were used as materials. The following trials will be as key ones to be done: the effect of AhSAMS1 on the morphological structure of lateral branch, the determination of hormone content, changes of methylation level and gene regulation analysis, and also to elucidate the mechanism of calcium regulated branching development by peanut calmodulin binding protein S-adenosine methionine synthetase 1.

分枝数是花生重要的植物学性状，与花生产量间呈显著正相关，是促成花生高产的主要因素。目前，人们对植物分枝发育调控分子机制的研究多集中在拟南芥和水稻等模式植物上，对花生分枝的研究还所知甚微。本课题前期从花生中筛选到一个新的钙调素结合蛋白S-腺苷甲硫氨酸合成酶1（SAMS1），过表达该基因的烟草植株分枝数明显增加。以对照花生、过表达AhSAMS1及CaM4和SAMS1双价表达的烟草株系为试材，通过观察花生SAMS1对侧枝形态结构的影响、激素含量的测定、甲基化水平变化及基因调控机理的研究，阐明钙调素结合蛋白S-腺苷甲硫氨酸合成酶1介导钙离子调控分枝发育的分子机制。

分枝数是花生重要的植物学性状，与花生产量间呈显著正相关，是促成花生高产的主要因素。目前，人们对植物分枝发育调控分子机制的研究多集中在拟南芥和水稻等模式植物上，对花生分枝的研究还所知甚微。本课题前期从花生中筛选到一个新的钙调素结合蛋白S-腺苷甲硫氨酸合成酶1（SAMS1），过表达该基因的烟草植株分枝数明显增加。以对照花生、过表达AhSAMS1的烟草株系为试材，通过研究表明花生SAMS1促进亚精胺和精胺含量增多，维管柱排列整齐，腋芽发育完整度高，IAA运输能力增强，有利于增加分枝数。RNA-Seq分析发现，与WT株系相比，AhSAMS1转基因株系中纤维素合成酶、S-腺苷甲硫氨酸合成酶及激素相关基因的表达都发生了变化，甲基化差异显示多富集在细胞生物代谢途径，生物调控，组织结构和催化活性等过程，进一步证实了AhSAMS1通过调控物质合成，激素变化进而调控植物的分枝。