基于固相微萃取技术和代谢组学方法研究有机污染物在植物体内的代谢

The metabolism of organic pollutants is an important detoxication route in plants, and is closely related to the fate of organic pollutants in the environment. At present, metabolomics methodology is drawing more and more attention in the revelation of the unknown metabolism paths of organic pollutants in plants. However, the solvent extraction method, which is often used for the extraction of metabolites of organic pollutants, encounters drawbacks as it contains many steps and takes relatively long time, and a large amount of plants need to be used in studying kinetics of metabolism and individual variations will therefore be introduced in the results. The present project is to develop novel solid-phase microextraction (SPME) fibers with broad spectrum extraction performance, and then develop simple and economical methods for identifying the metabolites of organic pollutants and revealing the metabolism paths of organic pollutants in plants by combining SPME and metabolomics methodology. In addition, the present project is to develop methods for studying kinetics of metabolism of organic pollutants in different living plant tissues bases on in vivo SPME, which could reduce the consumption of plant samples and avoid the influence of individual variations. This project could be helpful for promoting the development and application of SPME, as well as promoting the study of paths and kinetics of metabolism of organic pollutants in plants.

有机污染物在植物体内的代谢是植物解毒的重要途径，同时也与有机污染物在环境中的归趋密切相关。目前，在揭示植物体内有机污染物未知的代谢路径方面，代谢组学方法的应用正受到人们越来越多的关注。然而，常用来萃取植物体内有机污染物代谢产物的溶剂萃取方法却面临着突出的不足，包括过程繁琐且耗时较长、在代谢动力学过程的研究中需耗费大量的植物样品且会引入个体差异的影响等。本项目拟开发新型的具备广谱萃取性能的固相微萃取（SPME）探针，结合SPME技术和代谢组学方法，发展简便、经济的鉴定有机污染物在植物体内的代谢产物的方法，探明有机污染物的代谢路径；同时基于SPME技术，建立植物组织中有机污染物代谢动力学过程的活体研究方法，从而减少植物样品的消耗，并避免个体差异的影响。本项目的开展对于SPME技术的进一步发展与应用、以及植物体内有机污染物的代谢路径与动力学过程的研究均具有重要的促进作用。

按照计划书内容，本项目开展了新型固相微萃取涂层制备及污染物代谢产物鉴定方法研究的相关工作。本项目共发展了6种新型固相微萃取涂层，同时提出了以非共价质谱探针简化有机污染物代谢产物筛查过程的新思路。本项目发展的固相微萃取涂层及代谢产物筛查方法有望促进污染物代谢过程的研究。本项目共在Anal. Chem.、Chem. Commun.、Anal. Chim. Acta等期刊上发表研究论文16篇，申请发明专利3项，培养（联合培养）研究生4人，主要工作业绩均超过计划书设定的目标。项目负责人获批国家自然科学基金优秀青年项目，受邀担任Chinese Chemical Letters编委、Advances in Sample Preparation顾问编委、Frontiers in Analytical Science-Environmental Analysis副主编、广东省质谱学会理事。