基于HO/CO通路探讨“调肝理脾方”改善非酒精性脂肪肝的机制研究

Nonalcoholic fatty liver disease (NAFLD) have increased incidence in China and it may progress to hepatitis and cirrhosis. Abnormal iron metabolism and oxidative stress were found in NAFLD. We found Heme oxygenase/carbon monoxide (HO/CO) was activated in liver diseases process and it participates in iron metabolism andantioxidant effect. Our study showed “Tiao gan Li pi” Decoction could alleviate NAFLD patient’s symptoms, but the mechanism is unclear. Some literatures reported several herbs of “Tiao gan Li pi” Decoction induced HO-1 expression. Therefore we hypothesize that “Tiao gan Li pi” Decoction have liver protective effect, may regulate iron metabolism and oxidative stress through HO/CO pathway. We will use fat cells and high-fat diet-induced NAFLD rat model, discuss “Tiao gan Li pi” Decoction treat NAFLD characteristic and molecular mechanism. We will provide an evidence for “Tiao gan Li pi” of traditional Chinese medicine treat NAFLD.

非酒精性脂肪肝（Non Alcoholic Fatty Liver Disease，NAFLD）在我国发病率逐年升高，可进展为肝炎、肝硬化。近年来研究发现脂肪沉积过程中存在铁调节异常及氧化应激。我们研究发现，血红素氧合酶/一氧化碳（heme oxygenase/carbon monoxide，HO/CO）通路在肝病过程中被激活并参与肝内铁代谢及抗氧化；“调肝理脾方”能改善NAFLD患者临床症状，但其机制不清。文献报道“调肝理脾方”中多味中药能诱导HO-1表达。因此，我们提出“调肝理脾方”可能干预HO/CO通路，调节肝内铁代谢和氧化应激改善NAFLD脂肪沉积，发挥保肝作用。本项目采用脂肪细胞和高脂饮食诱导NAFLD大鼠模型，探讨“调肝理脾方”治疗NAFLD的特点及分子机制，为“调肝理脾”法治疗NAFLD提供理论依据。