冠菌素介导ABA和MeJA信号提高大豆抗旱性的调控机制

Drought is a major constraint to the production and yield stability of soybean [Glycine max (L.) Merr. ] in china. It is an important problem for the sustainable production of soybean to improve drought resistance and increase the utilization efficiency of water resources in soybean cultivation. Coronatine (COR) is a similar structure and function of jasmonates which has low molecular weight and high biological activity. COR can markedly enhance drought tolerance in soybean, but litter is studied to explore its regulation mechanisms and signaling pathways. This project is planned to select soybean variety Zhonghuang 20 as the tested material. The sensitive differnce of COR promoted dought tolerance is analyzed between V3 and flowering stages, and ABA and MeJA accumulated dynamics and distribution are detected under drought stress. VIGS technology is used to create VIGS-GmJMT and VIGS-GmNCED soybean materials, which is used to explore timing of COR -induced ABA and MeJA accumulation insoybean explored to drought. Transgenic AtLOS5, VIGS-GmJMT, VIGS-GmNCED and receptor soybean material are reciprocally or self-grafted to reveal ABA and MeJA organ specific expression induced by COR under drought condition. RNA-Seq is used to discover differential expression genes, and qRT-PCR and VIGS is used to detect expression level of selected differential expression. Based at the above analyse, signal and biochemical pathway would be build to explore that COR regulates drought tolerance in soybean. The results would be reveal physiological and molecular characteristics and signal transduction mechanism of COR induced dought tolerance in soybean, and have very important theoretical basis for establishment of higher efficient bio-saving technology in soybean cultivation.

干旱是大豆高产可持续的主要限制因素，提高植株抗旱性和水分利用效率是其可持续生产急需解决的关键问题。冠菌素（COR）是与茉莉酸类化合物结构和功能类似的高生物活性的生长调节物质，显著增强大豆抗旱性，但其调控途径及机制缺乏研究。项目拟以大豆中黄20为材料，研究COR调节植株响应干旱生化特征与ABA和MeJA信号表达的关系，比较COR调控植株抗旱的生育时期敏感性与ABA和MeJA积累器官特异性的关联性；利用VIGS-JMT、VIGS-NCED和转AtLOS5材料，结合嫁接，研究COR诱导ABA和MeJA积累响应干旱的时序性和器官表达特异性；构建COR诱导植株响应干旱基因表达谱，解析COR诱导差异基因表达与ABA和MeJA信号关联性，阐明COR诱导植株抗旱性表达的信号转导途径。研究结果为揭示COR诱导大豆植株抗旱性表达的信号途径及调控机制提供理论依据，对建立大豆高效生物节水栽培技术具有重要理论价值。