基于肠道宏病毒组学研究祛瘀生新法调控溃疡性结肠炎的机制

Ulcerative colitis,(UC) is a chronic non-specific inflammatory bowel disease.Owing to the pathogens remains unclear, it can’t be radical cure..With the development of Metavirus technique in recent years, the research(Norman et al, Cell, 2015) shows that the virus is the second large species in the intestinal. The Enteric Virome, not only may have a direct effect on the intestinal immunological functions, also the bacteriophage can effect on the human gut bacteria, and produced many recessive effects. The research focus on the enteric virome maybe is the breakthrough way to explain the relationship between the genetic and environmental factors which caused the ulcerative colitis. Thereby research in the enteric virome to be one of the hottest methods in the world..Based on the clinical trials and elemental researches of use Chinese traditional medicine, Blood Stasis Removing and renewal therapy(Quyu Shengxin method), Our research will detecte the fecal samples of the ulcerative colitis (U.C) patients by using Metavirus method to exam the exchanges and effects of the Enteric Virome in U.C patients before and after Chinese medicine treatment, thus to provide the evidences of using blood stasis removing and renewal therapy(Quyu Shengxin therapy) to treat U.C both in pathological aspects and mechanism.

溃疡性结肠炎是一种慢性非特异性肠道炎性疾病，由于发病病因至今未明，因此尚无法通过药物根治。.近两年来宏病毒组学技术的发展，2015年美国（Norman et al，Cell）研究显示肠道除细菌外，病毒是占第二位的微生物群体，它不但对肠道免疫有直接影响，同时肠道病毒（噬菌体）对肠道细菌也有影响。研究人类肠道未知的新病毒、病毒（噬菌体）对肠道菌群的作用，可能是解开溃疡性结肠炎发病两大诱因：遗传因素和环境致病的关键突破口，由此成为当前研究的最新热点之一。.本项目以溃疡性结肠炎患者为研究对象，以宏病毒组学技术为切入点，在以往临床和对肠道免疫、肠道菌群改变研究的基础上，纵向深入观察不同病理变化阶段和中药治疗后的肠道病毒种类和数量变化以及对肠道菌群的影响。阐明“祛瘀生新法”在治疗溃疡性结肠炎患者的应用规律及物质基础，为发挥中医药治疗的临床优势提供理论依据。

病毒是占第二位的肠道微生物群体，它不但对肠道免疫有直接影响，同时病毒（噬菌体）对肠道细菌也有影响。研究人类肠道未知的新病毒、病毒（噬菌体）对肠道菌群的作用，可能是连接遗传和环境致病的原因。.本项目以溃疡性结肠炎患者为研究对象，以宏基因组学技术为切入点，在以往临床和对肠道免疫、肠道菌群改变研究的基础上，检测溃结患者及正常志愿者的粪便，观察溃结患者中药治疗前后的肠道病毒种类和丰度变化及对肠道菌群的影响。.结果显示溃结患者肠道内病毒中有尾噬菌体，菌群中厚壁菌门、变形菌门及梭杆菌门的丰度较正常人群组高。治疗后有尾噬菌体丰度下降，拟杆菌门、梭杆菌门及变形菌门丰度下降，厚壁菌门及放线菌门丰度上升，目水平的乳杆菌目及双歧杆菌目的丰度有上升，有显著差异的基因代谢通路是碳水化合物代谢和催化活性。.结论：祛瘀生新方能提高人体机体免疫功能（扶正，生新），调节肠道内环境，抑制病毒组（噬菌体）及相关菌群的致病作用（祛邪，祛瘀）。为中医运用“祛瘀生新”学术思想治疗溃疡性结肠炎提供理论基础。