基于自由基学说的大粒车前子多糖抗炎免疫调节及相关机制研究

Plantago asiatica L. has been widely used as an edible Chinese herbal. In the previous studies, we reported the process of extraction and purification along with the method to analyse the primary structure for polysaccharides from Plantago asiatica L. (PLP), which showed an immune stimulating properties. The excellent anti-inflammatory potential and free radical scavenging activity of PLP suggest it may have a dual-regulation effect associated with the free radical regulating activity. In this research, to investigate the mechanism of anti-inflammatory function of PLP both at cellular level and overall level, a lipopolysaccharide-induced inflammatory model will be set in mice and dendritic cells as well as relevant immune organs from the model mice will be studied respectively. Further, the relationship between PLP with immune-regulation activity and free radical level will be discussed in detail.The results of this study will provide a good exploration method to solve the scientific problems of the mechanism of action of polysaccharides in vivo.The result will also impel people turn unutilized polysaccharide into valuables by using modern biotechnology, which are very important for the overall thorough research of the seed of Plantago asiatica L.. This research will also make contribution to the study of local special resource in China.

大粒车前子为我国药食两用物品。本项目组前期已完成大粒车前子多糖的提取纯化及一级精细结构解析工作，并发现其具有较好的免疫增强功能。而其良好的抗炎及自由基清除能力提示大粒车前子可能具有免疫双向调节能力，且与其调节机体自由基水平相关。本项目拟通过建立内毒素类炎症小鼠模型，以树突状细胞及相关免疫器官为对象，从细胞及整体水平探讨大粒车前子多糖抗炎免疫调节的可能机制，并对大粒车前子多糖、免疫调节及机体自由基的关系进行探讨。本研究为解决"多糖在体内的作用机制"这一科学问题提供了很好的探索方法。另外，本研究成果可促使人们利用现代技术充分利用大粒车前子中的多糖组分，对全面深入研究大粒车前子的价值有着非常重要的意义，也对全面深入开发我国特色资源中的活性物质有着重要的参考意义，为我国特色资源的研究提供新的思路。

本项目通过建立内毒素类炎症小鼠模型，以树突状细胞及相关免疫器官为对象，从细胞及整体水平探讨大粒车前子多糖抗炎免疫调节的可能机制，并对大粒车前子多糖、免疫调节及机体自由基的关系进行探讨。研究发现PLP预处理可减轻内毒素炎症小鼠的炎性症状，可能是通过改善氧化与抗氧化平衡紊乱状态而发挥作用，而PLP抑制DCs及T细胞活化也是其发挥炎症免疫调节作用的重要机制之一。本研究为解决“多糖在体内的作用机制”这一科学问题提供了很好的探索方法。另外，本研究成果可促使人们利用现代技术充分利用大粒车前子中的多糖组分，对全面深入研究大粒车前子的价值有着非常重要的意义，也对全面深入开发我国特色资源中的活性物质有着重要的参考意义，为我国特色资源的研究提供新的思路。