microRNA的表观遗传学调控在BMP9诱导MSCs成骨分化中的机制研究

At present, Mesenchymal Stem Cells(MSCs) are comprehensivly used as seed cells in bone tissue engineering ,during the developing processes of the tissue engineering technology, it has become an important segment how to verify the mechanism of osteogenic differentiation, which is thought as important scientific issue.In recent years,with the attention of miRNAs on evolution and effect of disease in mammals,as a new mechanism of epigenetic regulation,miRNA has become a hot issue on the modern stem cells research field.Our research group found that miR expression profile has changed and is time dependence during the processes of MSCs osteogenic differentiation induced byBone Morphogenetic Protein9(BMP9)on early days,so we select differential expressive miR-155.Combined with bioinformatics analysis,we speculated it plays an significant role on MSCs osteogenic differentiation induced by BMP9 that DNA has been methylated (one of epigenetics ways) through by miR-155 involved. And therefore,our reaserch group decided to study the specific mechanism of miR-155 epigenetics regulation on MSCs osteogenic differentiation induced by BMP9.The successful execution of this project will further enrich and perfect the theroy of MSCs osteogenic differentiation induced by BMP9,and lay a foundation for final solving the important scientific problem about"miRNAs epigenetic mechanism in stem cells directional differentiation",furthermore,it promotes the application of BMP9 in clinical bone tissue engineering..

间充质干细胞（MSCs）是目前骨组织工程中广泛采用的种子细胞，探明其定向分化机制是组织工程技术发展的重要环节。近年来，随着对miRNAs在哺乳动物进化及疾病发生发展中作用的关注，miRNA作为表观遗传调控的新机制，已成为现代干细胞分化研究的热点问题。课题组前期研究发现，BMP9诱导MSCs成骨分化过程中，miR-155的表达存在差异，且呈时间依赖性。结合生物信息学分析，我们推测：miR-155的DNA甲基化修饰（表观遗传学方式之一）在BMP9诱导的MSCs成骨分化中发挥了很重要的作用。因此，本课题围绕BMP9诱导MSCs成骨分化中，miR-155的表观遗传学调控机制展开研究。该项目的成功实施将阐明miRNA表观遗传学调控在BMP9诱导MSCs成骨分化中的作用机制，为最终诠释"干细胞定向分化中的miRNAs表观遗传学机制"这一重要科学问题奠定基础，也将推动BMP9在临床骨组织工程中的应用。

间充质干细胞（MSCs）是目前骨组织工程中广泛采用的种子细胞，探明其定向分化机制是组织工程技术发展的重要环节。近年来，随着对miRNAs在哺乳动物进化及疾病发生发展中作用的关注，miRNA作为表观遗传调控的新机制，已成为现代干细胞分化研究的热点问题。课题组前期研究发现，BMP9诱导MSCs成骨分化过程中，miR-21,miR-23b,miR-155的表达存在差异，且呈时间依赖性。结合生物信息学分析，我们推测：miR-155的DNA甲基化修饰（表观遗传学方式之一）在BMP9诱导的MSCs成骨分化中发挥了很重要的作用。因此，本课题围绕BMP9诱导MSCs成骨分化中，miR-21,miR-23b,miR-155的表观遗传学调控机制展开研究。该项目的成功实施阐明了miRNA调控在BMP9诱导MSCs成骨分化中的作用机制，为最终诠释“干细胞定向分化中的miRNAs表观遗传学机制”这一重要科学问题奠定基础，也将推动BMP9在临床骨组织工程中的应用。