神经递质对肝细胞增生和代谢的影响

To explore the effects and mechanism of autonomic nervous control on the.proliferation of human hepatocytes. And to examine the cellular localization of some.related receptors expression in human hepatocytes..Methods: Different concentrations of Norepinephrine(NE), and its agonist,.antagonist, acetylcholine(ACh), and its antagonist have been added to human.hepatocyte line L02 and hepatoma line BEL7402 of different planting densities..Modified MTT assay was employed to test the effects of them on the proliferation.of the two cell lines at 4h ,24h,48h and 72h. Immuocytochemical staining was used.to examine the cellular localization of alpha1B-adrenoceptor(α1B-AR), β2-AR and.epidermal growth factor receptor(EGFR) expression in human hepatocyte line L02..Results: NE significantly increased the cell proliferative rate of the two cell line,.especially 4h.The effect is enhanced significantly with dose increased. while.propanolol had no effect on NE potentiated proliferation. There were no significant.differences in the groups of metaproterenol compared with the negative control. ACh.significantly increased the cell inhibitory rate of L02. Its effect is enhanced with.time and dose increased. Atropine and scoline attenuated the effect of ACh. Scoline.国家自然科学基金资助项目结题报告.5.alone inhibited hepatocyte proliferation. In immuocytochemical staining, there were.positive responses to α1B-AR, β2-AR and EGFR in all cultures. It was observed that.the responses which is granular corpuscle to α1B-AR, β2-AR and EGFR were mainly.both cytoplasmic and cell membrane localized..Conclusions: NE, the sympathetic neurotransmitter, acting viaα1B-AR potentiate the.proliferation of human heatocyte and hepatoma in the presence of serum. ACh, the.vagus neurotransmitter, acting via mAChR and nAChR inhibit hepatocyte.proliferation.

肝脏富于神经分布，但其对肝细胞增生和代谢的影响尚未明了，本课题拟在体外肝细胞培养的条件下，从细胞生物学、生化及分子生物学角度研究不同的神经递质对生理及病理（肝硬化、肝癌）状态下肝细胞增生数量、ＤＮＡ合成、细胞受体变化以及细胞内多种酶活性的影响。揭示神经控制在正常生理状态下及肝硬化、肝癌发病机理中的作用。.