基于檀香诱导接香株模型研究檀香醇生物合成途径及调控机制

The heartwood of Santalum album L is rich in essential oil, which was used as traditional Chinese medicine. Only after ten years of growing can the essential oil be formed, which was commonly known as JieXiang. It was about thirty years' accumulation of essential oil that the heart can meet the standard for pharmaceutical use(≥3%). The mechanisms of JieXiang remain unknown, due to its long process and very few living cells in heartwood. Our team found the hormone could induce juvenile wood to produce Xiang in a comparatively short term, which its production was the same as the one produced in nature. The production speed of JieXiang and the amount of living cells also can meet experimental requirement of mechanism research. Base on the model, the biosynthetic pathway of santalol would be investigated with GC-MS method, according to the chemical changes in heartwood before and after induction. We would also analyze the differential expression proteins using the method of two-dimensional electrophoresis. In accordance with the corresponding changes of chemicals and biological macromolecules, we could illuminate the mechanisms of JieXiang and reveal reasons for slow process of it in natural state. The research would provide scientific basis to shorten the time and regulate the process of JieXiang, and provide innovative ideas for studies on secondary metabolic product of resin as well.

中药檀香为檀香富含挥发油的心材。该植物通常生长10年后心材才开始逐步形成挥发油（结香），积累至30年左右才能符合药用要求（挥发油≥3%）。由于受到结香进程缓慢，心材活细胞稀少等因素制约，其结香机理至今未明。本项目组发现生长素可在短期内诱导幼龄檀香提前结香，其结香产物与自然结香者相同，其结香速度与活细胞数量利于结香机理研究。为此，本项目以檀香挥发油中主要成分檀香醇（60-80%，g/g）为结香指标，以生长素诱导结香株为实验模型，应用GC-MS等手段分析诱导前后不同阶段心材化学成分变化，推测檀香醇的生物合成路径；并应用双向电泳等技术分析差异蛋白。通过结香过程化学小分子与生物大分子同期变化，初步阐明檀香结香机理，揭示自然结香缓慢原因，为有效调控檀香结香进程，缩短结香时间提供科学理论依据，也为树脂类药用植物次生代谢研究提供新的思路。

中药檀香为檀香富含挥发油的心材。该植物通常生长10年后心材才开始逐步形成挥发油（结香），积累至30年左右才能符合药用要求（挥发油≥3%）。由于受到结香进程缓慢，心材活细胞稀少等因素制约，其结香机理至今未明。本项目组发现生长素可在短期内诱导幼龄檀香提前结香，其结香产物与自然结香者相同，其结香速度与活细胞数量利于结香机理研究。为此，本项目以檀香挥发油中主要成分檀香醇（60-80%，g/g）为结香指标，以生长素诱导结香株为实验模型，应用GC-MS等手段分析诱导前后不同阶段心材化学成分变化，推测檀香醇的生物合成路径；并应用双向电泳等技术分析差异蛋白。通过结香过程化学小分子与生物大分子同期变化，初步阐明檀香结香机理，揭示自然结香缓慢原因，为有效调控檀香结香进程，缩短结香时间提供科学理论依据，也为树脂类药用植物次生代谢研究提供新的思路。