壳聚糖微纳米纤维表面改性及皮肤创面再生修复研究

The skin wounds clinical case is about 6 million a year on the world. And the incidence of chronic skin wounds about trauma 1.5%-3% of clinical cases, the cure rate of less than 50%, left double pain to body and spirit for the patients, cause a huge burden to family and society, so it is an important scientific problem. The project is the development of the biomaterial micro engineering design for the regeneration and repair of chronic skin wounds, the chitosan micron / nano fiber mixture will be used as the base materials, taking chemical modification with hydrophilic groups (hydroxybutyl, Carboxymethyl, quaternary-N, Etc.) on the surface of the chitosan fiber, to get a shallow layer gel coating on the fiber surface, then a fiber / gel integrated new wound dressing biomaterials will be get. This new dressing materials keep the fiber mechanical strength and has a moist and flexible, can absorb wound effusion and no further injury for the wound, do not hurt new granulation tissue of the wound, can maintain air permeability, maintain the antibacterial activity, promoting wound healing properties of chitosan, and it is a new dressing materials for chronic wound healing and health repair. In this project, the skin cells and cytokines will be combined with the biomaterials to research the influence for chronic wound repair from the factors of the biomaterials structure and micro environment. The cell attachment, proliferation, differentiation, secretion and wound healing effects will be studies to elucidate the relationship of ' structure & function ' of the biomaterials. The result will create the clinical utility of chronic wound dressing and has important significance for biomaterials design and tissue engineering technology.

每年皮肤创伤达600万临床病例，慢性创面占1.5%-3%，但治愈率不足50%,给患者肌体和精神留下双重痛苦，是急需解决的重要科学问题。本项目针对于皮肤创面再生修复，开展生物材料微工程设计，用壳聚糖微米纤维/壳聚糖纳米纤维混合基底，采取表面亲水基团化学修饰（羟丁基、羧甲基和季铵盐等），实现纤维浅表层凝胶化镀层，研制出纤维/凝胶的一体化新型生物材料。改性后的材料保持优良好力学强度，又具有湿润性和柔性，能吸收创面的积液和无再损伤换药，不伤及新生肉芽组织，保持良好的透气性，保留了壳聚糖的有抑菌性、促进伤口愈合等特性，得到有利于创面，特别是慢性创面快速愈合和健康修复的新型敷料。进一步将材料与皮肤组织细胞\细胞因子相结合开展实验研究，探索结构因素和微环境因素对皮肤相关细胞的贴附、增殖、分化、分泌和伤口愈合过程等的影响，阐明生物材料‘结构～功效’关系，对生物材料制造和再生医学均具有重要意义。

本项目针对于皮肤创面的再生修复特点，以壳聚糖纤维为基底，完成了生物材料微工程设计。采取表面亲水基团化学修饰，实现纤维浅表层凝胶化镀层，研制出纤维/凝胶的一体化新型生物材料：羧甲基表面修饰壳聚糖纤维（CM-FB&gel）、羟丁基表面修饰壳聚糖纤维（HB-FB&gel）、琥珀酰基表面修饰壳聚糖纤维（SA-FB&gel）和季铵盐基表面修饰壳聚糖纤维（QA-FB&gel）。新材料保持了优良力学强度，又具有湿润性和柔性，能吸收创面的积液，实现无再损伤换药，不伤及新生肉芽组织，保持良好的透气性，保留了壳聚糖的止血、抑菌、促进伤口愈合等特性。其中QA-FB&gel对金黄色葡萄球菌的抑制率大于99.9%、CM-FB&gel纤维组的创面愈合面积达到约100％，新材料表层的水凝胶保留了均一的三维网络结构，凝胶中的孔径为5-10um，为细胞的生长分化提供了湿润的三维网络环境和通透性，研究成果对生物材料制造和再生医学均具有重要意义。