剑叶龙血树内生菌的分离及其活性代谢产物研究

Endophytes of medicinal plants are sources of abundant biologically active material. Firstly, the endophytic actinomycetes and fungi will be isolated and preserved from their host Dracaena cochinchinensis (Lour.), which is one of the most important sources of dragon’s blood-a deep red resin famous in traditional Chinese medicine (TCM) and is mainly distributed in Xishuangbanna, Yunnan province. Secondly, the talent strains will be identified and their fermentation conditions will be optimized according to the screening of functional genes combined with bio-activities and chemical diversities. The compounds will be isolated in fermented exacts of the talent strains by chromatography and the chemical structures of purified compounds will be elucidated through spectroscopic analyses. Subsequently, bioactivities of all purified compounds, such as the antimicrobial and cytotoxic activities will be evaluated. Finally “Endophytes of D. cochinchinensis-Optimum fermentation conditions of talent strains-Secondary metabolites-Bioactivities” database will be constructed. The correlations between endophytes and the host D. cochinchinensis will be analyzed by comparing the difference of metabolites derived from endophytes and their host plants. This study will discover active ingredients and leading compounds, lead to an alternative approach to resolve such problems as the wild resources shortage of D. cochinchinensis, and it could further develop the traditional Dai medicines to contribute to human health.

药用植物内生菌中蕴藏着丰富的生物活性物质。项目以云南西双版纳主产的少数民族药用植物剑叶龙血树为研究对象，对其内生放线菌、真菌进行菌种分离和保藏，通过功能基因筛选、生物活性跟踪结合化学多样性确定目标菌株及其最适发酵条件，应用各种色谱手段对菌株发酵产物进行系统分离和纯化，以现代波谱技术和化学手段阐明单体化合物的结构；对所获单体成分进行抗菌（多种病原细菌、真菌及临床耐药菌）和抗肿瘤细胞等生物活性评价，建立“剑叶龙血树内生菌－发酵条件－次生代谢产物－生物活性”数据库；比较内生菌代谢产物与宿主主成分的异同，探讨内生菌与宿主植物代谢机制的相关性。本研究可为现代药物创新提供活性成分和先导化合物，为解决传统药用植物剑叶龙血树野生资源紧缺等问题提供了新的方向，也是推进“傣药”产业健康发展有益的探索。

药用植物内生菌中蕴藏着丰富的生物活性物质。项目以云南西双版纳主产的少数民族药用植物剑叶龙血树为研究对象，从其茎、叶组织中分离保藏了内生放线菌304株，内生真菌345株；通过功能基因筛选、生物活性跟踪结合化学多样性优选出45株目标菌株并确定其最适发酵条件；应用各种色谱手段对菌株发酵产物进行系统分离和纯化，共得到153个单体化合物；以现代波谱技术和化学手段确定了90个化合物的结构；对所获单体成分进行抗菌和抗肿瘤细胞等生物活性评价，得到数个具有显著抗菌或细胞毒活性的化合物。本研究可为现代药物创新提供活性成分和先导化合物，为解决传统药用植物剑叶龙血树野生资源紧缺等问题提供了新的方向，也是推进“傣药”产业健康发展有益的探索。