昆虫葡萄糖6磷酸脱氢酶基因结构和序列进化的比较研究

The total of 1200 base pair sequences of exon 2,3,4,and intron 2 and 3 of Glucose-6-phosphate dehydrogenase gene from 15 insect species were amplified using nested PCR technique and then directly sequenced via BIGDYE sequencing kits. The primary analysis results show that both exons and introns of the Glucose-6-phosphate dehydrogenase gene are extremely conserved during insect evolution. The phylogenetic relationships of tested insects were constructed through analyses of DNA sequences and their translated protein sequences. This is the first study on dehydrogenase gene family from comparative genomics perspectives, and the data provide important clues to understand gene structure organization and sequence evolution of dehydrogenase gene family. Beside sequencing and analysis of Glucose-6-phosphate dehydrogenase gene, we also conducted the following studies:.(1).The origins and evolution of insect-specific genes and gene families using comparative genomics and bioinformatics tools.(2)We also conducted comparative mitochondrial genomics using long-distance PCR technique amplifying whole mitochondrial genome.(3)We also applied the Expert System methods to the taxonogy of China Acridoidea, and designed the new software -- Expert System of Classification, Retrieval and Diagnosis for China Acridoidea (ESCA), as an effective tool of Classification Retrieval and Diagnosis in our country's manage and survey of the grasshopper disaster.

通过四对简并引物采用改进的巢式PCR 技术扩增并测出30种昆虫G6PD基因的3个外显子和2瞿诤拥男蛄性?200bP。用计算机分析这些DNA序列及其翻译出的相应蛋白质序列，探讨该基因在昆虫纲中的进化特征及保守内含子的可能调节功能，并构建昆虫纲30个目间的系统发育关系。具有重要的意义。.