胶原样蛋白在细菌-植物根系相互作用中的生态学功能研究

Rhizosphere biofilm covering the root surface is an important way of its interaction with plant roots; the coverage of bacterial biofilm on the root surface is a complex process that multi-extracellular matrix components of bacteria and cell surface components of plant co-participate in. This project is intended to focus on Bacillus amyloliquefaciens as the research object. It will be revealed the importance of bacterial adhesion protein to the formation of biofilm through the expression of bacterial collagen-like protein gene and microstructure changes in different growth stages of the plant roots; It intends to clarify the role of the collagen-like protein-coding genes by transcription initiation sites, acting elements, regulatory factors and signaling pathways, and to find the existence of the bacteria - Plant signaling pathway in the rhizosphere microenvironment, and then to clarify the mechanism from the molecular level; It will be probed the importance of the bacterial adhesion proteins in extracellular matrix components for microorganisms - plants contemplated, by the study the ecological functions of the collagen-like protein in the whole process of the attachment, colonization and mature biofilm of B. amyloliquefaciens in rhizosphere microenvironment ; This project will split resolution and microscopic study the entire ecological processes of interaction between rhizosphere microorganisms and plant roots, and will provide a theoretical basis for some important applications using bacteria such as biological control, promoting plant growth and improving plant stress-resistance.

根际细菌生长形成生物被膜覆盖在根表面，是其与植物根系相互作用的重要方式；生物被膜对根表面的覆盖是一个有多种细菌胞外基质成分和植物细胞表面成分共同参与的复杂过程。本课题拟以解淀粉芽孢杆菌为研究对象，通过对在植物根系相互作用中不同生长阶段下的胶原样蛋白的表达分布和相关显微结构变化的研究，揭示粘附蛋白成分对形成生物被膜的重要性；拟通过对胶原样蛋白编码基因的转录起始、作用元件、调控因子及信号通路的研究，发现根际微环境中存在的相关细菌-植物间信号通路，从分子水平阐明其作用机制；拟通过研究根际微环境下解淀粉芽孢杆菌的附着、定殖到成熟生物被膜的整个过程中胶原样蛋白的生态学功能，阐明细菌胞外基质成分中粘附蛋白对于微生物-植物相互作用的重要性。本课题将对根际微生物-植物根系相互作用的整个生态学过程进行拆分解析和微观研究，为利用细菌进行生物防治、植物生长促进、提高植物抗逆能力等重要应用方向提供理论依据。

根际细菌生长形成生物被膜覆盖在根表面，是其与植物根系相互作用的重要方式；生物被膜对根表面的覆盖是一个有多种细菌胞外基质成分和植物细胞表面成分共同参与的复杂过程。该项研究主要以解淀粉芽孢杆菌为研究对象，对在植物根系相互作用中不同生长阶段下的胶原样蛋白基因的表达分布和相关显微结构变化进行了分析，揭示了粘附蛋白成分对细菌形成生物被膜的重要性；对胶原样蛋白编码基因的转录起始、作用元件、调控因子及信号通路的研究，发现根际微环境中存在的相关细菌-植物间信号通路的作用机制；根际微环境下解淀粉芽孢杆菌胶原样蛋白具有附着、定殖到成熟生物被膜的整个过程的生态学功能，阐明细菌胞外基质成分中粘附蛋白对于微生物-植物相互作用的重要性。本课题将对根际微生物-植物根系相互作用的整个生态学过程进行拆分解析和微观研究，为利用细菌进行生物防治、植物生长促进、提高植物抗逆能力等重要应用方向提供理论依据。