富含半胱氨酸的类受体激酶StCRK1和StCRK2在马铃薯抗病防御反应中的功能研究

Potato (Solanum tuberosum) is the fourth-largest food crop in the world. Inner Mongolia is the largest potato-producing area in China. Potato plants are susceptible to a wide variety of diseases. Recently cysteine-rich receptor-like kinases (CRKs) are found to be involved in the plant defense responses. The proposed research will focus on two CRKs in potato named StCRK1 and StCRK2 which can be induced by Erwinia carotovora and salicylic acid analog. Their subcellular localization and tissue-specific expression will be investigated. Their expression level after pathogen and phytohormone induction will be studied. To elucidate their fuctions in defense responses and which hormone pathways they are involved in, overexpression and silencing StCRK1/2 plants of potato will be generated to study their disease resistance, reactive oxygen burst, and expression level for marker genes of hormones. And yeast two hybrid will be performed to find out the interaction proteins for StCRK1/2，which will pave the way for further research on the signal transduction pathways for StCRK1/2. The results of our research will help to explore the innate immunity in potato and to utilize it for disease resistance.

马铃薯是世界第四大粮食作物，内蒙古是我国最大的马铃薯产区，生产中马铃薯的病害非常严重。富含半胱氨酸的类受体激酶（CRK）近年来被发现参与了植物的抗病防御反应，马铃薯中还没有任何一个CRK的功能得到阐明。本研究将针对马铃薯中能被软腐病病原菌和水杨酸类似物诱导表达的两个CRK基因StCRK1和StCRK2，明确其亚细胞定位情况及组织表达特异性；受病原菌和激素诱导表达的情况；通过研究过表达和沉默转基因植株的抗病性能、活性氧爆发及激素标记基因的表达情况，明确其在抗病防御反应中的功能及参与的激素路径；并通过酵母双杂交确定与其互作的蛋白，为后续研究其信号传导途径奠定基础。本研究将为阐明马铃薯自身的免疫反应，利用其自身防御机制提高抗病性提供帮助。

马铃薯是世界第四大粮食作物，内蒙古是我国最大的马铃薯产区，但病害一直困扰着马铃薯的生产。富含半胱氨酸的类受体激酶（cysteine-rich receptor-like kinase, CRK）近些年被证明参与了植物的各种生物和非生物胁迫。本项目研究了马铃薯中两个CRK-StCRK1/2的亚细胞定位情况，明确了StCRK1/2定位于细胞膜上；主要在马铃薯的叶片中表达；可以受水杨酸（SA）、茉莉酸（JA）、脱落酸（ABA）和乙烯的诱导表达，接种致病疫霉及低温和高盐处理后也会诱导StCRK1/2的高表达；通过酵母双杂交技术获得与StCRK1互作的2个蛋白，均为含有U-box结构域的E3泛素连接酶，与StCRK2互作的1个蛋白，为输入蛋白（importin）类α2亚基；利用农杆菌介导的转化获得了由诱导型启动子驱动的StCRK1转基因马铃薯植株，发现其可增强对马铃薯青枯病原菌的抗性。上述研究证明了StCRK1/2参与了马铃薯的抗病防御反应，丰富了马铃薯自身内源免疫的机理研究，为后续进一步利用植物的内源免疫生产抗病品种奠定了基础。