短花针茅荒漠草原土壤微小动物群落特征及其分子生态网络对气候变化的响应机制

To lay the foundation for the study of soil microfauna community at the molecular level, here, we want to make clear the corresponding relationship between the results of morphological identification and molecular identity of the micro fauna in desert steppe soil. Thus, the corresponding library of soil micro fauna (including soil nematodes, mites and collembola) morphological identification and molecular identity will be constructed using molecular biology and morphological taxonomy. The experiment plots in stipa breviflora desert steppe will be performed which have been located with long-term artificial warming and drought simulation. To answer the response mechanism of soil microfauna community composition and diversity of the desert steppe to climate change when it becomes drier and warmer, the specialized species and sensitive species which have strong reaction to climate change will be locked by using the method of high-throughput sequencing and morphological identification. Based on the results of high throughput sequencing and the relative abundance data of the gene sequence OTU in the soil under different climate change gradients, the molecular ecological network of soil microfauna will be constructed. Further to evaluate the effects of exogenous disturbance caused by climate drying and warming on molecular ecological network stability of soil microfauna community. In this part, we will focus on the locations and their link of soil microfauna specialized species and sensitive species which have strong reaction to climate change in the molecular ecological network, further to reveal their interaction and coexistence when dealing with climate change.

在自然放牧的短花针茅荒漠草原，模拟气候变化（变干、变暖）长期定位试验的基础上，利用分子生物学、形态分类学等研究方法，构建土壤微小动物（包括土壤线虫、螨虫及弹尾目）的形态学鉴定与分子身份对应文库，明确荒漠草原土壤微小动物形态学鉴定结果与其分子身份之间的对应关系，为在分子水平上研究土壤微小动物群落奠定基础；采用高通量测序技术和形态学分类鉴定的方法，明确土壤微小动物的群落组成及多样性对气候变化的响应机制，锁定对气候变化反应强烈的土壤微小动物敏感种及特化种；应用高通量测序结果，基于不同气候变化梯度下土壤微小动物的基因序列OTU的相对丰度数据，构建土壤微小动物分子生态网络，评价气候变干、变暖的外源干扰对土壤微小动物群落分子生态网络稳定性的影响，重点分析对气候变化反应强烈的土壤微小动物敏感种及特化种在分子生态网络中的位置及关联性，揭示荒漠草原土壤微小动物群落应对气候变化的互作共存关系。

在自然放牧的短花针茅荒漠草原，模拟气候变化（变干、变暖）长期定位试验的基础上，利用分子生物学、形态分类学等研究方法，构建了土壤微小动物（包括土壤线虫、螨虫及弹尾目）的形态学鉴定与分子身份对应文库，明确了荒漠草原土壤微小动物形态学鉴定结果与其分子身份之间的对应关系，为在分子水平上研究土壤微小动物群落奠定了基础；采用二代测序技术和形态学分类鉴定的方法，明确了土壤微小动物的群落组成及多样性对气候变化的响应机制，确定了对气候变化反应强烈的土壤微小动物敏感种及特化种；应用测序结果，基于不同气候变化梯度下土壤微小动物的基因序列OTU的相对丰度数据，构建了土壤微小动物分子生态网络，评价了气候变干、变暖的外源干扰对土壤微小动物群落分子生态网络稳定性的影响，重点分析了对气候变化反应强烈的土壤微小动物敏感种及特化种在分子生态网络中的位置及关联性，揭示了土壤微小动物群落应对气候变化的互作共存关系。本项研究发表论文12篇，SCI收录8篇，其中TOP期刊3篇；培养硕士研究生10名；研究成果在国内外学术会议上展示交流40余人次。