艾灸调节免疫抑制效应的作用机制

Abstract To investigate the molecular mechanism of moxibustion in modulating immunosuppression induced by H22-bearing BALB/C mice through endogenous cytokines from Gene transcription to protein expression in CNS and immune target cells gene transcription，cytokines content molecular diversification in peripheral blood serum in mice. We studied on the endogenous immune cytokines IL-1,IL-2,IL-6 and signal transduction pathway Jak-Stat mediated by IL-2R, and Stat5a, Stat5b-important transcriptional factor of immunomodulation, upregulated different subunits of IL-2R and Stat5a, Stat5b mRNA to directly effect target gene transduction and protein expression, improve IL-1?、IL-2 mRNA expression and level of IL-1?、IL-2 of target tissure, dynamic changes of IL-1,IL-2,IL-6 in cytokines network in CNS participate in the modulation of neruo- endocrine- immunological network, to regulate the Immunosuppression state positively.

通过采用分子杂交、电泳技术与免疫学方法，观察艾灸调节肿瘤诱导的免疫抑制?信号系统的缺陷，及其与内源性细胞因子的关系；观察艾灸诱导的内源性细胞因子在中嗌窬低车木繁泶铩Ｍü难逵胝Ｑ宓缬竞?HPLC 对比分析，对艾灸血清有无斐；蛱匾煨员浠谐醪椒治觥１狙芯恳韵赴蜃游行模又惺嗌窬低?、免疫器官⒘馨拖赴托藕糯枷低?，揭示内源性细胞因子是艾灸调节免疫抑制效应的重要机制。从神经－内分泌－免疫调节网络揭示针灸治病原理。.