生长素运输载体PGP和AUX家族调控水稻根系发育的分子机制

Polar auxin transport regulates root system development. In dicotyledonous plant Arabidopsis thaliana, three gene families are involved in auxin transport: efflux carrier, pin-formed (PIN ) and P-glycoprotein ( PGP ); influx carrier, auxin resistant 1/like AUX1 ( AUX ). The PIN family has been widely studied, but for the investigation of the both families PGP and AUX, that is still not perfect; especially in monocot rice, their role is completely unknown. This project by using the auxin transport carriers OsPGPs and OsAUXs of Tos17 or T-DNA insertion mutants and overexpression materailsin rice,and analyzing phenotypic and physiological characteristics, research on the biological functions which participate in auxin transport,distribution and absorption of mineral nutrients; research on relationship between auxin transport carrier families, response to other plant hormones and the regulation of root system development. The study significances are in revealing the mechanism of rice root development with the molecular level, in providing theoretical basis of the root system improvement and root shaping for the related cereal crop and in establishing the foundation for breeding the high yield, high quality of rice varieties.

生长素极性运输调控根系发育。在双子叶植物拟南芥中，三个基因家族参与生长素的运输：输出载体（efflux carrier），pin-formed（PIN）和 p-glycoprotein (PGP)；输入载体（influx carrier），auxin resistant 1/like aux1 (AUX)。PIN家族已被广泛研究，但对PGP和AUX家族的研究尚不完善，尤其在单子叶植物水稻中的作用完全未知。本项目通过分析水稻生长素运输载体（OsPGP和OsAUX）的Tos17或T-DNA插入突变体、超表达材料的表型和生理特征，研究它们在生长素运输、分布及在矿质营养吸收中的生物学功能;研究运输载体各基因家族互作关系，对其它植物激素的响应及对根系发育的调控等。研究意义在于从分子水平上揭示水稻根系发育机制,为相关禾谷类作物的根系改良和根型塑造提供理论依据,为培育高产、优质水稻品种奠定基础。

生长素极性运输调控根系发育。在双子叶植物拟南芥中，三个基因家族参与生长素的运输：输出载体（efflux carrier），pin-formed（PIN）和 p-glycoprotein (PGP 或ABCB)；输入载体（influx carrier），auxin resistant 1/like aux1 (AUX)。PIN家族已被广泛研究，但对ABCB和AUX家族的研究尚不完善，尤其在单子叶植物水稻中的作用完全未知。本项目通过分析水稻生长素运输载体（OsABCB14和OsAUX1）的Tos17或T-DNA插入突变体、超表达材料的表型和生理特征，研究它们在生长素运输、分布及在矿质营养吸收中的生物学功能;研究运输载体各基因家族互作关系，对其它植物激素的响应及对根系发育的调控等。研究意义在于从分子水平上揭示水稻根系发育机制,为相关禾谷类作物的根系改良和根型塑造提供理论依据,为培育高产、优质水稻品种奠定基础。本研究揭示了生长素调控水稻根系生长发育的分子机理：首次发现了ATP Binding Cassette B（ABCB）14是生长素输入载体，可为MOGS所N-糖基化修饰进而调控根系发育；首次发现AUX1/LAX（OsAUX1）基因对水稻主根和根毛延伸的调控及和耐镉的关系，揭示了生长素信号新的基因调节网络。发表国际TOP期刊SCI IF>6的论文5篇，其中一篇为ESI高被引论文。项目负责人被国际著名期刊Plant Journal、New Phytologist、Plant Cell and Environment邀请为审稿人。