电针对肝癌小鼠生物节律的影响及其表观遗传学机制研究

。Keeping normal circadian rhythms are the most important condition which can keep body healthy. The disruptions of circadian rhythms are closely connected with the development and treatment of cancer. Regulating the pathological rhythm can alleviate the clinical symptoms and improve the quality of life and the survival of patients. Acupuncture is two-ways functional regulation therapy. As non-optical guidance factor, acupuncture can regulate various physiological and pathological rhythms of human bodies. On the basis of previous studies and document analysis, our research are aimed at the importance of epigenetic clock genes on the development of cancers, forming the hypothesis of acupuncture regulating the circadian rhythms and cell cycle rhythms through repairing the epigenetic variation of clock genes of cancer patients. Our research are targeted at epigenetic mechanism of electro acupuncture the acupoints of Ganshu(BL18) and Zhiyang(DU9) regulating the biological rhythms of liver cancer and cell cycle rhythms under the guidance of the TCM theories by selecting reliable chronobiology methods, DNA methylation biochip, bisulfite genomic sequencing, chromatin immunoprecipitation assay(ChIP) and other techniques and taking mice bearing liver cancer as the observing targets. It has important academic values? for popularizing the apply of acupuncture in clinical for preventing cancer and other diseases connected with rhythm disruption, inheriting and developing of the traditional chronomedicine such as midnight-noon and ebb-flow.

维持正常的昼夜节律是机体健康所必需，昼夜节律紊乱与癌症发生、发展及治疗、预后密切相关，积极调整癌症患者病理性节律可减轻临床症状，提高生活质量，改善预后。针灸立足于整体功能双向调节，针刺是一种对机体多种生理病理节律有调整作用的非光性授时因子。本研究在前期研究及文献分析基础上，针对生物钟基因的表观遗传改变在肿瘤发生发展中的重要性，形成了"针刺可通过调节生物钟基因表观遗传的变异实现对肿瘤生物节律及细胞周期节律调节"的工作假说，拟以荷H22肝癌小鼠为对象，采用时间生物学方法、DNA甲基化生物芯片、基因组测序和染色质免疫共沉淀(ChIP)等表观遗传学技术，研究在中医理论指导下电针肝俞、至阳穴调整肝癌及肝癌化疗小鼠生物节律及细胞周期的表观遗传学机制，为促进针灸用于临床防治癌症等节律紊乱密切相关疾病提供生物学基础和科学依据，对继承、丰富和发展"子午流注"等中医传统时间医学理论的科学内涵具有重要学术价值。

维持正常的昼夜节律是机体健康所必需，昼夜节律紊乱与癌症发生、发展及治疗、预后密切相关，积极调整癌症病理性生物节律可减轻临床症状，提高生活质量，改善预后。针灸立足于整体功能双向调节，针刺一种对机体多种生理病理节律均有调整作用的非光性导引因子。本研究在前期研究及文献分析基础上，针对生物钟基因的表观遗传改变在肿瘤发生发展中的重要性，形成了“针灸可通过调节生物钟基因表观遗传的变异实现对肿瘤生物节律及细胞周期节律调”的工作假说，拟以荷 H22 肝癌小鼠为对象，采用时间生物学方法、DNA 甲基化生物芯片、重硫酸盐基因组测序和染色质免疫共沉淀(CHIP)等表观遗传学技术，研究在中医理论指导下电针肝俞、至阳穴调整肝癌小鼠及化疗小鼠生物节律及细胞周期节律的表观遗传学机制，为促进针灸用于临床防治癌症等节律紊乱密切相关疾病的生物学基础和科学内涵，为继承、丰富和发展“子午流注”等中医传统时间医学理论具有重要的学术价值