基于高灵敏荧光共振能量转移生物传感器的环境雌激素致DNA氧化损伤修复响应机理研究

Environmental estrogen enters human body through food chain. It catabolizes and interferes the mechanism of DNA oxidative damage and repair in vivo, inducing mutation and cancer cells. Currently, the study on this mechanism shows disadvantages of complex operation, time consuming and high cost. In the project, nanoporous alumina membranes are modified by carbon quantum dots and immobilized with DNA. Environmental estrogen catabolizes and causes oxidative damage to DNA. It is labeled by gold nanoparticles modified with specific antibody. Combined with stable emission of carbon quantum dots and broad absorption spectral of gold nanoparticles, a fluorescence resonance energy transfer (FRET) biosensor is constructed based on nanoporous alumina membrane with carbon quantum dots and gold nanoparticles acted as the donors and acceptors. The FRET biosensor is used to investigate fluorescence response mechanism and sensing properties of DNA oxidative damage caused by environmental estrogen catabolism and base excision repair. Based on carbon quantum dots modified nanoporous alumina membrane, we develop a novel FRET biosensor with high sensitivity and specificity. It provides a new way for studying DNA damage and repair mechanism caused by environmental estrogen.

环境雌激素通过食物链进入人体分解代谢，破坏体内DNA氧化损伤修复机制，可诱发基因突变和细胞癌变。目前对该机制的研究操作普遍复杂、耗时、成本高。本项目将以表面功能化碳量子点修饰的氧化铝纳米多孔膜为基底，在碳量子点表面固定DNA。环境雌激素代谢引起DNA氧化损伤，利用表面特异性抗体修饰的金纳米颗粒对损伤DNA进行标记。结合碳量子点稳定的发光特性和金纳米颗粒较宽的吸收光谱特性，拟构建基于纳米孔膜，以碳量子点-金纳米颗粒为荧光供体-受体对的新型的高灵敏荧光共振能量转移（FRET）生物传感器，用于研究环境雌激素代谢引起DNA氧化损伤及其碱基切除修复的荧光响应机理和传感特性。该项目立足于碳量子点修饰的氧化铝纳米多孔膜，发展了一个新的高灵敏和高特异性的FRET生物传感技术，可用于分析研究环境雌激素代谢引起DNA 损伤修复机理，为DNA损伤修复研究提供了新方法和新途径。

环境雌激素通过食物链进入人体分解代谢，破坏体内DNA氧化损伤修复机制，可诱发基因突变和细胞癌变。目前对该机制的研究操作普遍复杂、耗时、成本高。本项目构建基于碳量子点和金纳米颗粒的荧光共振能量转移生物传感器，用于DNA氧化损伤生物标记物8-羟基脱氧鸟苷的高灵敏度特异性检测，具有较高的灵敏度和较低的检测限。将表面功能化的碳量子点修饰到氧化铝纳米多孔膜上，在碳量子点表面固定DNA。环境雌激素代谢引起DNA氧化损伤，利用表面特异性抗体修饰的金纳米颗粒对损伤DNA进行标记。结合碳量子点稳定的发光特性和金纳米颗粒较宽的吸收光谱特性，构建基于纳米孔膜，以碳量子点-金纳米颗粒为荧光供体-受体对的新型的高灵敏荧光共振能量转移生物传感器，用于研究环境雌激素代谢引起DNA氧化损伤及其碱基切除修复的荧光响应机理和传感特性。该荧光生物传感器具有高灵敏、低成本等优势为DNA损伤和修复机制的研究提供新方法。