五种云南民族药用植物内生链霉菌中的新奇结构化合物及其生物活性研究

The resource of plants for ethnomedicine were abundant in yunnan province,and many secondary metabolite with novel structures were isolated from it.This medicinal plants existed many kinds of endophytic Streptomyces, and some Streptomyces have developed the abilities to produce same or similar bioactive substances as or to what their host plants produce.The ratio of new compounds was high in biological metabolism of Streptomyces.Many novel bioactive compounds were found from Streptomyces in adaptive clture condition, which have important value to the pharmaceutical research.Five Streptomyces were targeted by bioactivity and chemical screenings in 80 strains from ethnomedicine plant in yunnan province. The research of bioactive secondary metabolite from this 5 Streptomyces will be carried out to find novel active compounds.15-20 new compounds including of 2-3 unprecedented skeleton compounds and 4-5 bioactive constituents will be found through this project.The optimizations of fermented condition will be conducted on the metabolic pathway of strong active compounds to concentrate target molecule for the research of structure-activity relationship, more new compounds will be otained by inducing biotransformation of metabolite in microorganism, and the biosynthesis pathway of new metabolites will be also investigated. 4-6 SCI papers will be published.

云南省民族药用植物资源丰富，从中分离得到了大量结构新颖的活性成分，而在植物中存在着大量的植物内生链霉菌，有些链霉菌具有了产生与宿主植物相同或相似的生物活性物质的能力。链霉菌生物合成新化合物的比例高，在适当的培养条件下能产生结构新颖的次生代谢产物，并且大多具有多种的生物活性，在医药方面具有重要的应用价值。我们对从云南民族药用植物中分离得到的80种链霉菌进行活性和化学筛选，发现其中的5种植物内生链霉菌的代谢产物较多、活性较好。我们重点对这5种链霉菌进行次生代谢产物及其生物活性的研究。从中发现结构新颖活性较好的化合物。通过本项目的研究发现新化合物15-20个，包括2-3个骨架新颖的化合物和4-5个活性较好的化合物。对其中的强活性化合物进行发酵条件的优化，富集目标分子进行构效关系的研究，并通过微生物的生物转化行为，诱导新代谢物的产生，并探讨其生物转化途径。发表高水平SCI论文4-6篇。

我们从9种植物内生链霉菌、2种Nocardiopsis和8种真菌 。包括 Streptomyces sp. YIM 66142，Streptomyces YIM 64018，Streptomyces sp. YIM67005，Nocardiopsis terrae YIM 90022，Aspergillus sp., Streptomyces sp.，Streptomyces sp. YIM61470，Nocardia sp.YIM 64630，Streptomyces sp. T009，Streptomyces sp. T019，Penicillium sp. YIM PH 30003，Chaetomium globosum，Trichoderma sp. YIM 30002，Streptomyces sp. YIM 66142，Stachybotrys sp. PH30583， Streptomyces sp. ，Microsphaeropsis arundinis ，Aspergillus sp. Y-12，Penicillium sp. 中分离得到310个化合物，其中新化合物和新天然产物42个和部分活性化合物。发表SCI期刊论文25篇和1篇核心期刊论文。已毕业6名硕士研究生和3名博士研究生。