武夷山国家自然保护区凋落枯枝暗色丝孢真菌分类研究

Wuyishan national nature reserve, located in the subtropical region of southeast China, which has a complex landform and vegetation type, accumulates a thick dead branches layer to be a rich habitat for fungi, which contains a wide range of rich and specific dematiaceous hyphomycetes. However, these dematiaceous hyphomycetes on dead branches are still much unexplored in the national natural reserve of Wuyishan, and lack systematic and comprehensive investigation and taxonomy at genera and species level. Through plenty sample collection of dead branches from Wuyishan, based on the traditional taxonomy, combining the morphology, scanning electron microscope and molecular phylogenetics, we are going to do the species diversity research on genera and species of dematiaceous hyphomycetes on dead branches from Wuyishan: 1) Excavating the diversity and the distribution of genera and species of dematiaceous hyphomycetes on dead branches from Wuyishan; 2) Exploring most suitable artificial substrate and condition for geminating and growing, and based on morphology and molecular phylogenetics, evaluating and erecting the taxonomic criterion and classification framework of genera and species of dematiaceous hyphomycetes on dead branches; 3) Redefining the taxonomic status of many dubious genera and species, clarifing the disorder among those genera and species, rebuilding the taxonomic keys, and improving the taxonomic theory at genera and species level. All these results could enrich the dematiaceous hyphomycetes in our country, and provide experience accumulation for other taxonomic study of dematiaceous hyphomycetes in other different regions.

武夷山国家自然保护区位于亚热带东南沿海，地形地貌复杂，植被种类繁多，地表积累了丰富的适于真菌生存繁衍的凋落枯枝基质，真菌类群种类多样，但目前为止对本区域凋落枯枝基质上暗色丝孢真菌类群的挖掘贫乏，更缺乏对本类群物种的系统性调查和分类研究。本申请拟通过广泛征集武夷山国家自然保护区凋落枯枝标本，立足经典分类，综合种菌形态学、电镜技术和分子系统学手段，系统开展该地区凋落枯枝基质暗色丝孢真菌多样性研究：1）挖掘该地区凋落枯枝暗色丝孢真菌物种多样性及其分布；2）探索暗色丝孢真菌适于萌发生存的人工培养条件，利用形态学与分子系统学相结合的方法评价或建立部分属、种的形态学划分标准与分类框架；3）重新界定部分疑难属、种的分类地位，澄清若干属、种分类地位混乱，重建属、种分类检索表，完善属、种分类理论。该研究将丰富我国暗色丝孢真菌资源，为开展其它区域内暗色丝孢真菌分类研究提供经验积累。

武夷山国家自然保护区位于亚热带东南沿海，植被种类繁多，丰富的凋落枯枝基质孕育多种多样的真菌。但前人对本区域该种基质暗色丝孢真菌类群的系统性分类研究甚少。本项目通过广泛征集该地区凋落枯枝标本，借助形态学分类特征与分子系统学分析，系统开展该种基质暗色丝孢真菌多样性研究：1）探索暗色丝孢真菌适于萌发生存的人工培养条件，利用形态学与分子系统学相结合的方法评价或建立部分属、种的形态学划分标准与分类框架；2）重新界定部分疑难属、种的分类地位，澄清若干属、种分类地位混乱，重建属、种分类检索表，完善属、种分类理论；3）充分挖掘该地区凋落枯枝暗色丝孢真菌多样性，描述鉴定暗色丝孢真菌属45个，物种111个，其中新种13个，新记录种11个，中国已报道种87个，发表或预发表SCI期刊论文5篇，获得山东省自然科学二等奖1项，计算机软件著作权3项；4）该研究丰富了我国暗色丝孢真菌资源，为开展不同生态区域真菌物种多样性及其与植物多样性协同生存机制研究提供了经验借鉴。