多功能纳米二硫化钼光电化学传感探针对食品中赭曲霉毒素检测新方法研究

Photoelectrochemical (PEC) immunosensor is a newly emerged yet dynamically developing technique for various promising application including food safety detection, environmental monitoring and clinical diagnosis. Importantly, photoactive materials act as the key factor to the analytical performances of the photoelectrochemical immunosensors. MoS2 nanomaterial, a semiconducting analogue of graphene, shows great promise for photoactive materials owning to its attractive and unique photoelectric properties. The aim of the project is to explore and construct new methods and strategies for the analysis and detection of ochratoxin in foodstuff by using multifunctional MoS2 nanomaterials as efficient PEC nanoprobes. Detailedly, the project consisted of the following issues: (i) to construct a novel photoelectrochemical immunosensor for the monitoring ochratoxin based on graphene/MoS2 nanocomposites coupled with tyramine signal amplification; (ii) to explore multiple signal-amplified photoelectrochemical detection strategies by coupling competitive-type displacement reaction mode with exciton-plasmon interactions; (iii) to exploit new competitive photoelectrochemical biosensing approach by using MoS2 nanocluster sensitized TiO2 nanoparticles. More importantly, this project will provide a useful tool for various scientific research fields relating to national production and public security (e.g. agricultural and animal husbandry production, food safety analysis, import and export commodity inspection, etc.).

光电化学免疫传感是一种新兴的检测技术，在临床诊断、环境检测和食品安全等诸多领域中有着广阔的应用前景。选择和制备性能优异的光电活性材料是建立高效光电化学免疫检测方法的关键所在。类石墨烯二硫化钼纳米材料以其独特的光电性质展现出作为光电活性材料的巨大潜能。本项目将合成多功能层状MoS2纳米材料作为光电化学传感探针，建立对食品中低浓度赭曲霉毒素检测的新方法和新技术。具体内容：(1) 建立以石墨烯/MoS2纳米复合物作为光电转换探针、酪胺信号放大技术为信号增强策略的新型光电化学免疫传感分析方法；(2) 建立基于竞争置换模式和激子-等离子体激元反应的新型光电化学免疫分析方法；(3) 建立以MoS2纳米簇敏化的TiO2纳米颗粒作为光电转换探针新型竞争型光电化学生物传感分析方法。本研究成果有望在我国农牧业生产、食品安全分析和进出口检验等国民生产和公共安全密切相关的科学领域发挥重大的作用。

灵敏和快速实现复杂体系中真菌毒素检测在临床诊断、环境检测和食品安全等诸多领域都有着重要的意义。电/光电化学生物传感分析方法以其优异的特性成为食源性真菌毒素检测的利器。信号放大对于电/光电化学生物传感体系的构建至关重要。本项目将铂增长诱导底物循环放大策略，酶催化产物腐蚀光电活性材料等信号放大技术引入至真菌毒素检测平台建立了一系列电化学和光电化学生物传感检测新技术和新方法。具体内容：(i) 基于酶催化产物腐蚀表面等离子体光电活性材料的新型光电化学免疫传感体系用于赭曲霉毒素A的检测研究; (ii) 基于二硫化钼纳米催化剂信号放大的电化学DNA传感器的构建; (iii) 基于双重信号放大的新型光电化学生物传感体系用于赭曲霉毒素A的检测研究； (iv) 基于原位铂增长介导底物循环放大新型电化学免疫传感器用于食品中赭曲霉毒素A的检测; (v) 基于即时催化剂放大的电化学免疫传感体系用于赭曲霉毒素A的检测研究。该项研究实现了对复杂体系红酒样品中赭曲霉毒素A的初步检测应用，为进一步商业化提供了理论依据。