高灵敏羧基化苯胺/苝环类NMOFs信号标签的可控合成及电化学适体传感研究

Controllable, multi-functionalized and easy preparation for signal tags is the key to construct the highly sensitive electrochemical aptasensors. This project aims to develop the controllable synthesis of nanoscale metal-organic frameworks (NMOFs) as signal tags, which combines the merits of electrochemical activity, catalytic activity and carriers, and employs to apply in electrochemical aptasensors. Based on coordination chemistry, the electrochemical active ligand (such as carboxyl functionalized aniline derivatives or perylene derivatives, etc.) coordinates with metal ions leading to NMOFs, which is expects to achieve the controllable synthesis for NMOFs both in composition and structure. The structure and morphology of the as-prepared NMOFs would be obtained by SEM, TEM, XRD and single crystal X-ray diffraction analysis. The synergistic enhancement of metal catalytic active sites and ligand in NMOFs could be investigated in order to select signal tags with the high electrochemical stability and catalytic activity. The MOFs signal tags are to employ in labeling with avian influenza disease associated aptamers. In combination with nucleic acid signal amplification technology, a novel and efficient electrochemical aptasensors system would be established. Research results would provide a new idea for the rational design of signal tags with controllable synthesis, and present a new method for the construction of electrochemical biosensors based on NMOFs signal tags.

标记量可控、多功能且易于合成的信号标签是构建高灵敏电化学生物传感器的关键。本项目旨在制备一种电化学活性、催化活性及载体于一体且标记量可控的纳米金属有机骨架（NMOFs），并以此作为信号标签用于高灵敏电化学适体传感器研究。选取具有电化学活性的羧基化苯胺/苝环类配体与金属离子作用形成NMOFs，以配位为导向，以期实现NMOFs信号标签组成和结构的可控合成。通过SEM、TEM、XRD及X-射线单晶衍射技术获悉NMOFs的结构和形貌信息；研究NMOFs中金属催化活性位点与配体电信号的协同增强作用，筛选出电化学信号稳定和催化活性高的NMOFs信号标签；进一步用于标记禽流感疾病相关适体，结合核酸信号放大技术，建立界面组装简单可控、电化学信号稳定的新型高灵敏电化学适体传感新体系。研究结果为制备标记量可控的信号标签提供了新思路，同时为以NMOFs信号标签构建电化学生物传感提供了新方法。

标记量可控、多功能且易于合成的信号标签是构建高灵敏电化学生物传感器的关键。本项目旨在制备一种电化学活性、催化活性及载体于一体且标记量可控的纳米金属有机骨架（NMOFs），并以此作为信号标签用于高灵敏电化学适体传感器研究。选取具有电化学活性的配体与金属离子作用形成NMOFs，以配位为导向，制备得到系列三苯胺MOF、Fc-Zn-MOF、Ce-MOF、NH2-MIL-88、NH2-Ni-MOF和PtNi@MIL-101。通过SEM、TEM、XRD及X-射线单晶衍射技术获悉NMOFs的结构和形貌信息；研究MOFs对电信号分子的催化作用，筛选出电化学信号稳定和催化活性高的NMOFs信号标签；进一步用于标记疾病相关适体，结合核酸信号放大技术，建立界面组装简单可控、电化学信号稳定的新型高灵敏电化学适体传感新体系。研究结果为制备标记量可控的信号标签提供了新思路，同时为以NMOFs信号标签构建电化学生物传感提供了新方法。