携带CD-UPRT双功能基因的内皮祖细胞载体靶向治疗恶性胶质瘤的实验研究
基本信息
结项摘要
恶性胶质瘤是人类难治性肿瘤，也是血管形成最显著的肿瘤之一。近年研究证明，在肿瘤血管形成过程中，从骨髓基质动员而来的内皮祖细胞(EPC)可以自引导参与肿瘤血管发生并可进入肿瘤间质。本研究把EPC同高效率的酶/前体药物系统结合起来，立足于临床试验治疗的需要，分离、培养、扩增人外周血EPC,将胞嘧啶脱氨酶（CD）和尿嘧啶磷酸核糖基转移酶(UPRT)双功能融合基因转导入EPC；在裸鼠恶性胶质瘤颅窗可视模型和脑实质肿瘤模型中，颈动脉输注基因修饰的EPC后，按时间序列腹腔注射5-氟胞嘧啶（5-FC）；通过在体显微荧光录像系统、共聚焦荧光显微镜、动态核磁共振成像等方法,对肿瘤血管密度及组织病理形态、动物行为学及生存期变化等的观察，研究具有组织趋向性的EPC载体/CD-UPRT/5-FC治疗系统对恶性胶质瘤肿瘤血管形成及肿瘤实质的影响，并进行EPC体内安全性研究。
项目摘要
项目成果
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数据更新时间：2023-05-31
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