酸性氨基酸对慢性氧化应激断奶仔猪肠道微生物菌群干预机制研究

The development in aquaculture industry brings out the harm of stress intensifying. From improvement of animal health during pig breeding, alleviating oxidative stress becomes a key problem, which is also an urgent scientific issue in the field of animal nutrition. At the present status of pig breeding process, chronic stress is imperceptible but harmful to the intestine health of the pigs under intensive rearing. In response to this problem, we will establish a model of chronic oxidative stress to reveal the response mechanism of gut microbiota to oxidative stress and the relationship between oxidative stress and gut microbiota in weanling piglets. Using the techniques of microbiology and metabonomics, intestinal microbial community, as well as the feedback intervention of acidic amino acids and chronic oxidative stress will be analyzed systematically. This project will provide a new theoretical basis for the nutrition research and feeding management of piglets, with improvement of the immune function and alleviation the growth inhibition caused by oxidative stress. It has timely significance for the sustainable development of pig breeding.

随着养殖业发展所带来的应激危害日趋突出，探讨缓解氧化应激对生猪养殖业所造成的危害，提高生猪健康水平已成为动物营养学研究领域迫切需要解决的关键科技问题。本项目针对生猪生产过程中，慢性应激表征不易察觉、对集约化饲养的生猪肠道健康危害极大的现状，通过构建断奶仔猪慢性氧化应激模型，利用微生物组学和代谢组学等技术，系统的对肠道微生物群落进行解析，从而揭示肠道微生物菌群对氧化应激的响应机制，以及氧化应激与肠道菌群多样性的关系，并揭示断奶仔猪慢性氧化应激酸性氨基酸营养干预机制。本项目的开展将有益于增强仔猪的免疫机能，缓解氧化应激引起的生长抑制，能够为仔猪的营养研究与饲养管理提供新的理论依据，对生猪养殖的可持续发展具有重要的现实意义。

随着养殖业发展所带来的应激危害日趋突出，探讨缓解氧化应激对生猪养殖业所造成的危害，提高生猪健康水平已成为动物营养学研究领域迫切需要解决的关键科技问题。本项目首次采用D-半乳糖诱导断奶仔猪氧化应激，从而构建了断奶仔猪慢性氧化应激模型，发现慢性氧化应激影响了断奶仔猪结肠微生物多样性；通过解析慢性氧化应激断奶仔猪营养利用效率，发现氧化应激使断奶仔猪粪能和尿能损失增加，同时机体内总能和净能之间的转化率降低，抑制了断奶仔猪对饲料中能量的利用，降低了氮沉积、氮表观消化率和氮生物学价值，提高了氮排放；探讨了天冬氨酸对仔猪断奶后不同时间点小肠微生物定植、分布和功能演变的规律，阐明肠道菌群多样性与宿主天然免疫应答的关系，揭示天冬氨酸调控断奶仔猪肠道微生物菌群与黏膜天然免疫应答的关联机制。本项目的开展将有益于增强断奶仔猪的免疫机能，缓解氧化应激引起的生长抑制，能够为仔猪的营养研究与饲养管理提供新的理论依据，对生猪养殖的可持续发展具有重要的现实意义。