大麦苗粉和籽粒特高蛋白质含量及其营养功能成分QTLs分析

It is an important approach of functional barley for the chronic disease prevention of human being; however pearl barley with shell and its grass powder is very important on human health but poorly understood. High protein barley resource from Yunnan province, SSR markers and advanced detection technology are combined. We bred with 188 lines of RILs based on parents between Zaguangmangluoerlen of the world's highest protein content in grain (22.59% ) and Schooner of low protein content (9.80%) for China's import of the largest, the determination of 30 functional nutrition components in barley grains and its grass powder, which reveals their relation among functional nutrition components. Identification of quantitative trait locus for the highest protein and its amino acid content as well as its functional nutrition component-related content in grain and grass powder of barley. It is clear that QTLs locus differences and genetic variation characteristics for their relation among functional nutrition components, bred the new lines with the highest functional nutrition components contents in grain and grass powder of barley. It is not only conducive the development of promotion barley functional food, beer industry and animal husbandry as well as genetic molecular basis for functional nutrition components, but also is of great significance and high practical value to the implementation of new strategies for the disease prevention of diet and solved global sub-health problems.

功能大麦是预防人类慢性病的重要途径；然而大麦米及其苗粉对人体健康影响十分重要但知之甚少。发挥云南高蛋白质大麦资源优势、SSR标记和先进检测技术等相结合，以世界最高籽粒蛋白质含量的紫光芒裸二棱(22.59%)与中国进口量最大的Schooner(9.80%)杂交加代培育出188个株系的RILs及其亲本为材料, 测定籽粒及其苗粉30种营养功能成分并揭示大麦籽粒及其苗粉营养功能成分的关联性；定位紫光芒裸二棱籽粒特高蛋白质及其氨基酸含量和籽粒或苗粉特高营养功能成分的QTLs位点。明确大麦的籽粒和苗粉营养功能成分关联性的位点差异及其群体遗传变异特征和创新特高营养功能成分新材料。不仅有利于促进大麦功能食品、啤酒工业和畜牧业发展及其揭示营养功能成分间遗传的分子基础，而且对实施饮食预防疾病新战略解决全球亚健康问题具有重大意义和较高实用价值。

功能大麦是预防人类慢性病十分重要但知之甚少。该项目测定了紫光芒裸二棱 Schooner培育193个RILs株系及其亲本籽粒及其苗粉30种营养功能成分, 揭示了大麦苗粉/籽粒营养功能成分的关系及其关联性的分子基础,取得了如下重要进展：.1. 揭示了大麦RILs籽粒/苗粉的蛋白质、9种元素、4种功能成分及16种氨基酸含量的相关性、遗传变异特征、变种类型与营养功能成间的差异；培育出青稞籽粒/大麦苗粉高元素和特高功能成分优异种质18份。.2. 用均匀分布在大麦7条染色体604个SSR标记筛选出两亲本间多态性标记180个，在193个RILs进行扩增,构建了大麦7个连锁群180个SSR位点的分子连锁图谱,遗传图谱总距离2671.03 cM,间距14.84cM。.3. 定位大麦籽粒/苗粉的蛋白质、4种功能成分和8种元素及籽粒15种氨基酸酸共110个QTLs位点有55个主效位点。定位籽粒/苗粉蛋白质12个QTLs有5个主效位点,定位籽粒/苗粉4种功能成分27个QTLs有13个主效QTLs位点,定位籽粒/苗粉8种元素24个QTLs有10个主效位点;定位籽粒15种氨基酸47个QTLs有27个主效位点。.4. 发现了大麦籽粒/苗粉营养功能成分16个QTL簇：第7染色体GMS056 -GBM1297(9)>BMAG0914-GBM1464(7)>GBM1303-GMS056(4)>EBmac603-BMAG0914 (3)>HVCABG-EBmac713(2)=Bmac0218-Bmag0189(2)和BMAG0900-Bmag011(2)存在QTL簇；第1染色体scssr08238-Bmac213(4)>Bmag0211-GBMS0014(3)>Scssr04163- WMC1E8 (2) 存在QTL簇；第2染色体Scssr03381-Scssr07759(4)和Bmac0132- Bmag0571(2)存在QTL簇；第4染色体HVBAMMGB84-BMAG0808(8)和第6染色体Bmag0174–GBM 1270 (6)及第5染色体Scssr03907-GBMS0032(2)存在QTL簇。.5. 发表学术论文12篇,其中3篇SCI收录论文影响因子5.412和7篇核心期刊论文影响因子9.081。