毒物在毛发根部的动力学模式及其与血液、毛干毒物分布的关系

Hair analysis evidence has special value in the field of forensic science due to the retention mechanism of the poison in hair, and the hair root plays an important role in the clarification of the hair poison retention mechanism and the interpretation of the hair analysis, as the source of the poison into the hair. The study established the experimental model of toxicokinetics and poison distribution, to clarify the toxicokinetics of psychoactive poison in hair root. LC/MS/MS and MALDI-MSI were applied in the qualification, quantification and location of trace target compounds in the hair root. And the concentrations of poison and metabolites in the hair root, hair shaft and blood were detected and traced in animals with different dosage. The kinetics model of the target compounds in the hair root was established to obtain the concentration of poison hair root and blood and relationship to kinetic behavior, the changes of kinetic behavior of poison before and after death, and the relationship between the dynamic behavior and the distribution of the target in the hair shaft. The characteristics of drug information contained in hair root may illuminate the entry mechanism and retention mechanism of poison into hair from a new vision, clarify the practical value of hair root analysis in forensic toxicology and enhance the reliability of hair analysis evidence.

毒物在毛发中的保留属性赋予毛发分析在法科学领域的特殊证据价值，而毛根作为毒物进入毛发的源部位，对于毛发毒物保留机制的阐明以及毛发分析结果解释具有重要意义。本项目以毛根毒物动力学特征研究为基点，以精神类毒物为目标物，构建毒物动力学实验模型和毛发毒物分布实验模型，以及基于LC/MS/MS和MALDI-MSI的毛根中痕量目标物的定性、定量、定位技术；同步考察不同给药剂量下，毛根、毛干、血液中目标物和代谢物的存在情况及其随时间的变化；研究阐明毛根中目标物的动力学模式，建立毛根和血液目标物浓度以及动力学行为的关系；考察研究对象生前死后毛根中目标物动力学行为的变化及其与毛干中目标物分布的关系。以期以新的视觉探析毛发中毒物的进入机制和保留机制，揭示毛根所蕴含的摄毒信息特征，阐明毛根分析在法医毒物学领域证据价值和应用价值，并提升毛发分析证据的可靠性。

为拓展毛发分析在法医毒物学领域的应用范围和证据价值，本项目以毛根毒物动力学特征研究为基点，以精神类毒物咪达唑仑、喹硫平为研究对象，创新建立毛发微样中痕量毒物的LC/MS/MS和MALDI-MSI的定性、定量、定位分析技术，为精准识别毛根和毛干中毒物的保留、分布特征提供新的手段；创新同步研究毛根、毛干、血液中目标物和代谢物的存在情况及其随时间的变化，阐明毛根中目标物的动力学模式，建立毛根和血液、毛干目标物动力学行为的关系，以新的视觉探析毛发中毒物的进入机制和保留机制；首先揭示报道毛根所蕴含的摄毒信息特征，通过应用研究评估毛根分析的适用范围、信息特点和证据价值，推动毛根分析和全发分析在死后毒物学领域的应用。