环状RNA782作为竞争性内源RNA调控miR-200s在EMs上皮-间质转化机制中的作用

The pathogenesis of endometriosis is still unknown. Among many different theories, Sampson theory is the most widely accepted. Many scholars believe that epithelial-mesenchymal trasition (EMT) gives endometrial epithelial cells the abilities of invasion, metastasis and implantation，then promotes the formation of EMs. In the former work of our laboratory, we proved that the expression levels of hsa_circ_000782 in eutopic and ectopic endometrial tissues of EMs are different from that in normal tissues. However, its regulatory mechanism in EMs remains unclear. New research is showing that circRNA can affect the incidence of the disease by regulating miR-200s, which are associated with many diseases. So we speculate that by the way of being competitive source of RNA, circ_000782 may regulate the activity of miR-200s , and affect the process of EMT, thereby play a role in the occurrence and development of EMs. By using FISH fluorescence in situ hybridization technique and Luciferase report gene detection, we are supposed to find that hsa_circ_000782 can inhibit the activity of miR-200s by combining with it. Then we may investigate its specific signal conditioning path and verify the target gene effect. As a result, we can get the possible mechanism of how hsa_circ_000782 affecting the occurrence and development of EMs, which can provide new ideas and specific targets for the diagnosis, treatment and follow-up detection recurrence of EMs.

子宫内膜异位症（EMs）发病机制不明，目前以Sampson经血逆流种植为主导理论。很多学者认为上皮-间质转化（EMT）赋予了子宫内膜上皮细胞“侵袭”、“转移”和“种植”的能力，从而促进EMs的形成。 我们发现：环状RNA782在子宫内膜异位症中异位、在位内膜和正常内膜中具有高度差异性表达，但其在EMs中的调控机制尚不明确。最新研究提示circRNA可通过与疾病关联的miRNA相互作用，从而影响疾病的发生。因此我们推测环状RNA782以竞争性内源RNA方式抑制miR-200s的活性而促进EMT过程，从而促进子宫内膜异位症的发生与发展。本项目采用FISH荧光原位杂交技术与荧光素酶报基因检测，明确环状RNA782与miR-200s的结合并抑制其活性，继而探明其具体的信号调节通路，阐明circRNA782影响EMs发生和发展的机制，为EMs的诊断、治疗以及随访检测复发提供新思路和特异性靶点。

子宫内膜异位症（EMs）是一种慢性、进展性且临床表现多种多样的疾病，常见的症状有周期性痛经、慢性盆腔痛、月经失调、不孕等，严重影响女性的生活质量。我们通过研究证明了上皮-间质转化（EMT）在EMs发病机制中发挥这一定作用。在此基础上，我们又发现了在EMs组织中差异表达的circRNAs、lncRNAs、miRNAs （circRNA 0067301、 lncRNA CDKN2B-AS1、lncRNA BANCR、Linc-ROR、miR-424-5p、miR-141、miR-204），通过离体实验发现这些差异表达的非编码RNAs可通过ceRNA作用参与EMs相关EMT，并影响EMs细胞增殖、迁移、侵袭等生物学行为。研究中进一步探索围绕差异表达的非编码RNAs为EMs的诊断、治疗提供新思路和特异性靶点。