鼠尾藻光系统对重金属胁迫响应的分子机制探讨

Macroalgae were used to repair heavy metals polluted water, with significant and unique environmental and ecological benefits of low investment, high biomass, targeted, non-polluting, the adsorption efficiency, and had broad application prospects in the repair of offshore pollution. Algae had established a series of adaptive mechanisms in the process of long-term heavy metal stress response, and by controlling the absorption, enrichment, transport and detoxification of heavy metals, the different cellular components remained within the normal concentration range. Therefore, the study of response mechanism of algae to heavy metal stress is of great significance. In this study, Sargassum thunbergii under heavy metal stress and the normal thalli were chosen as study objects, the GeneFishing technical were employed to study the differentially expressed genes during the process of heavy metal stress, to find the key response genes of Sargassum thunbergii photosystem to heavy metal stress, and to cloning the whole sequence, dentification the function of expression. The completion of this project for proven the response mechanism algae to heavy metal stress further, and thus provide a theoretical basis for new species of algae cultured from the molecular level which were high tolerance, high enrichment of heavy metals.

利用大型藻类修复重金属污染水体，具有投资小、生物量大、针对性强、无污染、吸附效率高等显著而独特的环境生态效益，已在近海污染修复方面显示出广阔的应用前景。藻类在长期响应重金属胁迫的过程中，建立起一系列的适应机制，藻类通过控制重金属的吸收、富集、转运与解毒，使不同细胞组分中的重金属维持在正常浓度范围内。因此， 研究藻类对重金属胁迫的响应机理有着重要的意义。 本项目以重金属胁迫下的鼠尾藻及其正常藻体为研究对象，采用GeneFishing 技术对重金属胁迫过程中的差异表达基因进行研究，以找到鼠尾藻光系统响应重金属胁迫的关键基因，并对这些关键基因进行全序列克隆、表达等功能鉴定。本项目的完成为进一步探明藻类对重金属胁迫的响应机制，进而从分子水平上培养对重金属高耐性、高富集性的藻类新品种等研究提供理论依据。