基于线粒体蛋白质组学的益气法治疗慢性心力衰竭的机制研究

The Heart-Qi Deficiency Syndrome is the basic Pathogenesis of chronic heart failure. The disturbance of energy metabolic of cardiac myocyte is the basic pathological link of chronic heart failure.The former research suggested that there be close connection between The Heart-Qi Deficiency Syndrome and Energy metabolic disturbance.So we propose Nourishing Qi intervene in the disturbance of energy metabolic of exhausted cardiac myocyte by regulating proteomics of mitochondria. Refer to the common pathogeny of Chronic Heart Failure,We copy the rat model of CHF after myocardial infarction and abdominal aorta ligation,simulate the development of heart failure, mensurate the influence on proteomics of cardiac myocyte mitochondria and heart function by Nourishing Qi,try to clarify the influence on the disturbance of energy metabolic of cardiac myocyte by Nourishing Qi while looking for scientific evidence for the therapy of Chronic heart failure by nourishing Qi and expounding the substance of the Heart-Qi Deficiency Syndrome in terms of energy metabolism of cardiac myocyte.

心气虚是慢性心衰的基本病机，能量代谢障碍则是心衰基本的病理环节，既往研究也提示心气虚证与心肌能量代谢有密切的关系。因此，本研究提出益气法通过调控细胞能量工厂—线粒体相关蛋白质谱从而干预心衰心肌细胞能量代谢障碍的假说。参考心衰最常见的冠心病与高血压两种病因，复制心梗后心衰的大鼠模型与缩窄腹主动脉所致心衰的大鼠模型，模拟心衰的发展过程，利用双向电泳技术测定益气法对心力衰竭时心肌线粒体蛋白质表达谱及其对心功能的影响，从整体到功能蛋白的层次阐明益气法对心衰能量代谢障碍的干预作用，为益气法防治心衰提供科学依据，并通过益气中药的作用进行反证，从心肌能量代谢的角度，进一步阐明心气虚的实质。