新型细胞因子CX1的发现、免疫功能及其作用机理的研究

Cytokines play crucial roles in regulating the proliferation, differentiation and functions of a variety of cells. In this study, we investigated the biological activities of a novel cytokine named as CX1, which was origically isolated in our lab recently. Sharing homology to interleukin(IL)-17, cytokine CX1 promotes the proliferation of granulopoiesis and erythropoiesis, and stimulates the production of IL-6 and tumor necrosis factor (TNF-a) by monocytes. But it exhibits no obvious effects on dendritic cells or T lymphocytes. By expression cloning, the receptor for cytokine CX1 was identified, which encodes a type I membrane receptor of 502 residues. Furthermore, the Fc fusion protein for the extracellular domain of the receptor was successfully generated and functionally confirmed. Taken together, these data are valuable to better understanding the biological functions of cytokine CX1.

申请人已从人树突状细胞基因文库中克隆到与白介素17同源的新型细胞因子CX1（登录号为AF110385），并获得重组蛋白。本项目拟在此基础上研究CX1对树突状细胞体外分化发育、T赴鲋郴罨约暗ズ讼赴仔砸蜃雍捅泶锬け砻娣肿拥牡鹘谧饔茫⑻教制涫欠窆灿冒捉樗?7受体及其可能的信号传导途径，以阐明该新型细胞因子的免疫功能及其作用机理。