单宁诱导杨小舟蛾谷胱甘肽S-转移酶基因表达调控机制

The chemical relationship between insect pests and host plants is a very important part in insect pest management, and the adaptation of insect pests to the host plants dues to inducibility of their detoxification enzymes. Micromelalopha troglodyta (Graeser) is a very significant pest in poplar.It is useful to clarify for M.troglodyta how to utilize plant allelochemicals for increasing its adaptation. Therefore, the response element of tannin on promotor region of glutathione S-transferase (GST) gene will be identified and validated, and whether Fos/Jun (AP-1) complex binding to AP-1-like binding sites induced by tannin regluates the expression of MtGST gene in M. troglodyta will be investigated in this program.These results will demonstrate the expression reglutation mechanism of MtGST gene induced by tannin in M. troglodyta, which will make up the blank about the recept and conduction mechanism of herbivorous insects on plant allelopathy signal and be useful to clarify the resistant mechanisms and co-evolution of insect pest-host plant. Furthermore, c-jun and c-fos might be used as the target genes of RNA interference to control pests, and AP-1 complex might be potential new molecular target that is used to design and screen novel insecticides, which will provide the basis on the development of pest control technology and the discovery of new pesticides.

昆虫与植物间的化学关系是害虫治理的重要基础。解毒酶系的可诱导性是昆虫对寄主植物适应性的主要机制。杨小舟蛾是主要的林业害虫之一，明确杨小舟蛾如何利用植物次生物质作为感应信号提高其适应力，对于有效控制杨小舟蛾具有重要意义。该项目拟确定杨小舟蛾谷胱甘肽S-转移酶（GST）基因启动子区域单宁核心应答元件；探明单宁是否是通过诱导Fos/Jun异二聚体（AP-1转录因子）与AP-1结合位点结合调控杨小舟蛾GST表达。通过上述研究，明确单宁诱导杨小舟蛾MtGST基因表达调控机制，将弥补植食性昆虫感受和传导植物化感信号机制领域的空白，对于深入揭示害虫抗药性机制，植食性昆虫与植物的协同进化机制具有重要意义。此外，原癌基因c-jun和c-fos可能被用来作为防治害虫的RNA干扰的靶基因，同时AP-1转录因子可能成为潜在的新分子靶标，用来设计和筛选新型杀虫药剂，从而为发展杨小舟蛾控制新技术及新农药创制提供依据。

昆虫与植物间的化学关系是害虫防治的重要基础。解毒酶系的可诱导性是昆虫对寄主植物适应性的主要机制。杨小舟蛾是主要的林业害虫之一，明确杨小舟蛾如何利用植物次生物质作为感应信号提高其适应力，对于有效控制杨小舟蛾具有重要意义。该项目构建了单宁胁迫杨小舟蛾中肠及其对照测序文库，利用HiSeq2000技术进行了转录组测序，结果表明在转录组数据中寻找到GST差异表达最显著的基因64条（其中上调40条，下调24条）；克隆了10条杨小舟蛾GST基因，其中得到了其中5条GST的启动子全长；对比了单宁对杨小舟蛾GST各不同长度启动子活性的影响，明确杨小舟蛾GST基因启动子区域单宁响应元件；通过共转染研究了转录因子对杨小舟蛾GST表达的调控。通过上述研究，明确单宁诱导杨小舟蛾GST基因表达调控机制，弥补植食性昆虫感受和传导植物化感信号机制领域的空白，对于深入揭示害虫抗药性机制，植食性昆虫与植物的协同进化机制具有重要意义。此外，对于发展杨小舟蛾控制新技术及其新农药创制提供依据。