新的染色体易位t（11q13.2；19p13.2）在中国卵巢癌人群发病率及其临床病理分级和预后相关性研究

The incidence of ovarian cancer increased year by year, but its pathogenesis remains unclear, and there is also lack of early diagnostic marker for ovarian cancer, which caused poor prognosis and high mortality. In recent years, Some researcher found that the chromosomal abnormalities in ovarian cancer is very common, but the significance is still not clear. Our previous study found a variety of chromosomal rearrangements in ovarian cancer and the translocation between 11q13 and 19p13.13 is the most common abnormality.On the basis of our preliminary studies, we intend to use fluorescence in situ hybridization (FISH) to detect the translocation between 11q13 and 19p13.13 in the tissues of ovary,peripheral blood and ascites of patients who have ovarian cancer.We also intend to use the method of gene expression chip and RT-PCR to make certain the mechanism of expression products of the rearranging chromosome and its surrounding key functional genes on clinical ovarian cancer growth, metastasis and other biological behavior. We also want to make clear the effect of specific silence the fusion genes by the technology RNA interference on the biological behavior and apoptosis of ovarian cancer in vitro and in vivo(laboratory animals). By above-mentioned works ,we hope to make certain the marker chromosome of ovarian cancer and provide theoretical and practical basis for the early diagnosis of ovarian cancer and to explore new means of treatment.

卵巢癌发病率逐年上升，但其发病机制仍不清楚，且缺乏早期诊断标志，患者预后差，死亡率高。近年发现染色体异常在卵巢癌中比较常见，但其重要性尚不明确。课题组前期创新性的工作发现：卵巢癌组织存在多种染色体重排，而最常见的是存在染色体t（11; 19）（q13; p13.13）易位。课题组拟在前期研究基础上，应用荧光原位杂交（FISH）技术建立染色体t(11;19)(q13;p13.13)的组织、外周血和腹水检测技术，并通过基因表达芯片、RT-PCR等技术明确该易位染色体表达产物和其周边关键功能基因与临床卵巢癌发生、发展、转移等生物学行为的关系；最后通过RNA干扰技术特异性沉默该易位染色体所表达的融合基因，并通过体外和体内（动物）试验验证基因干预手段对卵巢癌生物学行为、凋亡等的影响。明确卵巢癌的标志染色体及其功能，为卵巢癌的早期诊断和探索新的治疗手段提供理论和实践基础。

卵巢癌发病率逐年上升，但其发病机制仍不清楚，且缺乏早期诊断标志，患者预后差，死亡率高。近年发现染色体异常在卵巢癌中比较常见，但其重要性尚不明确。课题组前期创新性的工作发现：卵巢癌组织存在多种染色体重排，而最常见的是存在染色体t（11;19）（q13; p13.13）易位。课题组拟在前期研究基础上，应用荧光原位杂交（FISH）技术建立染色体t(11;19)(q13;p13.13)的组织检测技术，并通过基因表达芯片等技术明确该易位染色体表达产物和其周边关键功能基因与临床卵巢癌发生、发展、转移等生物学行为的关系；最后通过RNA 干扰技术特异性沉默该易位染色体所表达的融合基因。明确卵巢癌的标志染色体及其功能，为卵巢癌的早期诊断和探索新的治疗手段提供理论和实践基础。