蛋白质多态性的的微型化学发光成像检测方法研究

This project studies the methodology of chemiluminescent (CL) imaging detection of protein phenotypes in polyacrylamide gels after electrophoresis. The sensitivity, selectivity and analytical speed were studied throughout the work. The method offers the advantages of rapid and simple procedures compared with the method of immunoblotting CL imaging currently presented in the literature. Several proteins, including haptoglobin (Hp) (including Hp1-1, Hp2-2 and Hp2-1), human serum albumin (HSA), as well as some unknown proteins, present in human serum have been sensitively detected by the proposed method using hemoglobin (Hb) and metal ions as probe. The CL imaging conditions including luminescence reagents, exposure time and probe concentrations have been optimized. Our results also showed that the separation could be greatly improved by using nanomaterials in gels. With the nanomaterials, some proteines that did not separated with normal gels have been well separated. This result indicated the potential of using nanomaterial in clinical and .biological electrophoresis..

本课题研究在纳米基质上进行微型电泳分离后进行蛋白多态性化学发光成像检测的新方法。研究内容包括基于蛋白亚单元直接化学发光反应进行微型蛋白斑点成像和研究以纳米基质的催化发光反应为背景进行成像的可行性,为微型凝胶电泳进行蛋白质多态性研究提供高灵敏鹊某上穹治鲂路椒ā