荔枝蒂蛀虫寄主保守选择的嗅觉分子机制

Conopomorpha sinensis Bradley (Lepidoptera: Gracillariidae) is a major fruit borer pest on litchi and longan in China. It causes serious damage to the fruit, even harm to the shoots and flowers. It is difficult to be controlled becauseof its better concealment. The eggs hatch and the larvae immediately penetrate the fruits, feeding on the seed neck. Even though the larvae effectively infect the fruits with a vast variety of microorganisms that eventually destroy the crop. It had caused significant economic loss and ecological disasters for the fruit export of chinese litchi and longan. Using the host plant volatiles to control C. sinensis is a green control technology with the characteristics of non-toxicity, non-pollution and sustainable control. However, a research of the connection between host plant volatiles and C. sinensis olfactory receptors, which restrict the development of this technology is still lacked. The molecular mechanism research of C. sinensis olfactory receptors in host-plant conservative choice will reveal the signal transduction pathways between host plant volatiles and C. sinensis olfactory receptors, and it will be a useful addition of the existing technology. This project takes the molecular mechanism of C. sinensis olfactory receptors as contents of study，and aims at explorations of olfaction signal pathway of C. sinensis. The effect and function of C.sinensis olfactory receptors (general odorant binding proteins, GOBP and chemosensory proteins, CSP) will be analysised according to the induction of host plant volatiles, electrophysiological technique and RNAi. The work will be carried out at a molecular level to identify the regulation expression and its pathway of C.sinensis olfactory receptors by host plant volatiles induced. And the the molecular mechanism of C. sinensis host-specificity will be clear. The research results will provide a technical support and theoretical basis for the pheromone control with the targets at the olfactory system of lepidoptera pests, and new ideas for the pollution-free production of litchi and longan.

荔枝蒂蛀虫是危害我国荔枝和龙眼生产的钻蛀性害虫，隐蔽性强，防治困难。利用寄主挥发物引诱或者干扰荔枝蒂蛀虫的防治技术具有无毒、无污染、可持续控制的优点，是一项极具潜力的绿色防治技术。而缺乏寄主挥发物和荔枝蒂蛀虫嗅觉受体之间联系的基础研究制约了该技术的发展，探讨荔枝蒂蛀虫寄主保守选择的嗅觉分子机制将揭开挥发物与嗅觉受体之间的信号传导途径，成为现有技术的有益补充。 本项目以荔枝蒂蛀虫嗅觉分子机制为研究内容，探索荔枝蒂蛀虫的嗅觉信号通路为目标，通过寄主挥发物诱导、电生理检测和RNAi干扰，研究和比较荔枝蒂蛀虫普通气味结合蛋白基因(CsGOBP)和化学感受蛋白基因(CsCSP)在荔枝蒂蛀虫寄主保守选择中的作用及功能，明确寄主挥发物对荔枝蒂蛀虫嗅觉受体基因的调控表达和传导途径，从而阐明荔枝蒂蛀虫对寄主保守选择的分子机制。研究结果可为以鳞翅目害虫嗅觉系统为靶标的信息素调控技术提供技术支撑和理论依据。

荔枝蒂蛀虫是危害我国荔枝和龙眼生产的钻蛀性害虫，隐蔽性强，防治困难。利用寄主挥发物引诱或者干扰荔枝蒂蛀虫的防治技术具有无毒、无污染、可持续控制的优点，是一项极具潜力的绿色防治技术。而缺乏寄主挥发物和荔枝蒂蛀虫嗅觉受体之间联系的基础研究制约了该技术的发展，探讨荔枝蒂蛀虫寄主保守选择的嗅觉机制将揭开挥发物与嗅觉受体之间的信号传导途径，成为现有技术的有益补充。本项目以荔枝蒂蛀虫嗅觉基因为研究内容，以探索荔枝蒂蛀虫的嗅觉信号通路为目标，通过寄主挥发物分析GC-MS、EAG测定、转录组和嗅觉相关基因分析，研究和比较了不同荔枝挥发物对荔枝蒂蛀虫寄主选择的行为，通过分子生物学技术研究嗅觉白基因(CsGOBP)在荔枝蒂蛀虫寄主保守选择中的作用及功能，明确了寄主挥发物对荔枝蒂蛀虫嗅觉受体基因的调控表达，阐明了荔枝蒂蛀虫对寄主保守选择的分子机制。研究结果为以鳞翅目害虫嗅觉系统为靶标的信息素调控技术提供技术支撑和理论依据。