基于聚集态纳米金的光热传感策略研究

Food safety issue is one of the major issues in China's livelihood at this stage. The development of fast, reliable, easy-to-operate and low-cost detection method which can enable the food safety issue be discovered earlier is also the key point to reduce losses, especially in the places where there are no professional testing institutions and large instruments. One of the common methods is visual method based on the color change resulting from the aggregation of gold nanoparticles. This method has the advantages of visual detection, easy operation, high response speed and so on, however, quantitative analysis by naked eyes is impossible and the sensitivity is relatively low. Consequently, given that the aggregated gold nanoparticles have photothermal effect in near infrared region and the high specificity between the recognition probe and target (such as aptamer and target), this project will propose different strategies that induces the aggregation of gold nanoparticles regarding different targets in food safety issue, and then develop a series of simple, efficient, low-cost and portable photothermal sensing strategies on the basis of aggregated gold nanoparticles, in which the thermometer is used as the quantitative signal reader, in this case, highly sensitive and quantitative analysis and visual qualitative analysis of the target can be achieved. This project can provide necessary technical support for the rapid detection of target regarding food safety issue and also extend the application areas of photothermal effect resulting from gold nanoparticles.

食品安全问题是现阶段中国民生领域正面临的重大问题之一。开发出快速可靠、操作简便、成本低廉的检测手段，使食品安全问题尽早发现，也是降低损失的关键所在，特别是在远离专业检测机构和大型仪器的情况下。当前常见分析手段之一是基于纳米金聚集以后颜色变化的可视化方法，该方法具有检测结果直观、操作简单、响应速度快等优点，但无法实现肉眼定量分析且检测灵敏度相对较低。本课题拟利用聚体态纳米金在近红外区具有光热效应的特性，结合识别探针与靶标分子的高特异性作用机制（如适配体与靶标分子），针对不同的食品安全问题目标物构建出不同的诱导纳米金聚集的方法，以温度计为定量信号读出装置，开发出一系列简单、高效、成本低且便携的基于聚集态纳米金的光热传感策略，通过温度与颜色的变化实现目标物的高灵敏定量和可视化定性分析。本项目可为食品安全问题的快速检测提供必要的技术保障，同时也拓展了基于纳米金光热效应的应用范围。

食品安全问题是现阶段中国民生领域正面临的重大问题之一。开发出快速可靠、操作简便、成本低廉的检测手段，使食品安全问题尽早发现，也是降低损失的关键所在，特别是在远离专业检测机构和大型仪器的情况下。当前常见分析手段之一是基于纳米金聚集以后颜色变化的可视化方法，该方法具有检测结果直观、操作简单、响应速度快等优点，但无法实现肉眼定量分析且检测灵敏度相对较低。本项目针对现有可视化方法存在的问题，以常见的温度计为定量信号读出装置，构建了一系列光热传感方法，主要研究内容如下：1）系统研究了聚集态纳米金光热转换效率的影响因素及相关机理，为后续光热分析方法的高灵敏性提供技术保障；2）针对不同种类的目标物构建出不同的诱导纳米金聚集的方法，以温度计为定量信号读出装置，开发了一系列简单、高效、成本低且便携的基于聚集态纳米金的光热传感策略；3）基于光热性能更好的钙钛矿纳米材料构建了高灵敏的光热传感方法，探讨了其光热转换效率的相关机理，拓展了光热传感方法的研究范畴。. 本项目完成了相关的研究目标，执行期间共发表高水平论文9篇，包括 Anal. Chem.(1篇)、Sensor. Actuat. B-Chem.(1篇)、Anal. Chim. Acta(3篇)、Talanta(1篇)、Nanoscale(1篇)、Microchim. Acta(1篇)、福州大学学报(1篇)，获得授权发明专利1项，培养博士研究生1人、硕士研究生3人。